多能干细胞基因组编辑：方法，误区和解决方案

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1月6日，Cell子刊《Cell Stem Cell》在线发表了来自哈佛大学和麻省总医院研究人员的一篇综述文章，题为“Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions”。
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  W( l: h6 R4 M0 vGenome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions2 o; w- z% J2 P9 m  P7 G
Summary: Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.' B, H6 U7 [; G: S$ h

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