慢病毒、腺病毒操作安全性问题

yaolinzhang

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如果做原癌基因，风险是存在的。建议楼主最好在生物安全柜中操作。

yaolinzhang

回复 lxy07 的帖子
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' F' T) B9 a7 o) ]原则上慢病毒操作应满足二级水平：
1 \! e& L5 Q9 m4 _# OSummary of Biosafety Level 2:
/ f  x7 r/ R% ?6 \Practices:
$ L2 X6 K, y& x) Y  Standard microbiological practices
1 q  j+ D; f0 N% D3 t+ l  Limited access to work area
( |1 ]! y8 v; `: L  Biohazard warning signs posted" i3 l* e, J& v) |1 V- p) W/ E
  Minimize production of aerosols! T! s0 @" a& ^' X7 u! W
  Decontaminate potentially infectious wastes before disposal
% \: \+ {, T4 F  Use precautions with sharps (e.g., syringes, blades)( c% Y# a$ v7 _% d( \- x( Q0 y
  Biosafety manual defining any needed waste decontamination or medical surveillance 2 I% @9 i; m, s7 S
policies
" A. t" G1 a$ W! x2 h4 K, e- A, gSafety equipment:
0 P$ [9 ~4 D5 M6 [9 V5 E  Biological Safety Cabinet, preferably a Class II BSC/laminar flow hood (with a HEPA
- g2 `. y, B1 C: D! Fmicrofilter) used for all manipulations of agents that cause splashes or aerosols of infectious , b3 S2 E. k5 S& h
materials; exhaust air is unrecirculated
- P; m4 {# v/ x9 k. j  PPE: protective laboratory coats, gloves, face protection as needed
" p2 W9 @* L* d% h0 {7 p9 i5 ]7 GFacilities:: g% l; ]$ F5 W2 L4 s( l
  Autoclave available for waste decontamination
* H( F3 N' e) E- r0 j  @  Chemical disinfectants available for spills9 x3 m: F) a' H; b
    不过总比超净工作台强，建议楼主将用过的枪头、培养液集中收集于装有84的容器内，密封。过夜后，高压处理即可。