神经干细胞可以用含血清的培养基吗? * u" g" V( G& G0 O6 u* c我最近看了一篇关于成体神经干细胞培养的外文文献,所用的培养液成分是:a mixture of 44 ml of Neurobasal (N medium (Gibco), 0.5ml of glutamine, 0.5 ml of B27 (Gibco), 5 ml of fetal calf serum, 0.04 ml of gentamicin(Cellgro) and supplemented with epidermal growth factor (EGF) (20ng/ml; Sigma) and fibroblast growth factor-2 (FGF2) (20ng/ml; Sigma)。他用了含10%胎牛血清的培养液来培养,但神经干细胞不是用含血清的培养液就会分化吗?文献后面讲到细胞分化时用的是15%胎牛血清的培养液,是不是在含10%胎牛血清的培养液中神经干细胞不会分化,而在含15%胎牛血清的培养液中就会分化啊?