有谁知道qPCR检测DNA文库浓度的原理是什么？

wangjintao

- e! e0 _& I& W0 Q  q6 N* F$ V/ ?! j# H' W9 S6 |5 X- `! [' G
有谁知道qPCR检测DNA文库浓度的原理是什么？

xuyang0317

用来定量的，测DNA浓度的方式偶很多。2100，qubit等。

lchanlon

Since q-PCR is designed to quantify the number of template. So you can use the standard library to quantify your libraries. I don't know for sure where your DNA libraries made from, what kind of kit you use for preparing the libraries and are for what kind of purpose? Here, i include a product data-sheet from Kapa Bio. Go through this sheet. You would find out what you want. I used this kit before. But I don't think it is a good protocol. For RNA-seq library quantification, I use PhiX library from Illumina. You can make your own serial dilution to generate a standard curve.

w343989328

构建好的DNA文库 两端都是相同的接头序列 根据这些接头序列设计引物就可以PCR了
! s: u2 v5 e9 P( A" O( m0 ]8 Z; N. i然后qPCR中有一套标准样品 根据样品CT值的大小实现定量