有谁知道qPCR检测DNA文库浓度的原理是什么？

lchanlon

Since q-PCR is designed to quantify the number of template. So you can use the standard library to quantify your libraries. I don't know for sure where your DNA libraries made from, what kind of kit you use for preparing the libraries and are for what kind of purpose? Here, i include a product data-sheet from Kapa Bio. Go through this sheet. You would find out what you want. I used this kit before. But I don't think it is a good protocol. For RNA-seq library quantification, I use PhiX library from Illumina. You can make your own serial dilution to generate a standard curve.