von kossa染色具体方法

tonyyang

vonkossa stain.pdf (13.05 KB)

2009-7-13 14:57 上传

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1. Sections to water. 切片脱蜡至水  
3 ?8 T3 b$ N: b6 T+ J2. Place sections in 1% silver nitrate solution under ultra- violet light for 45 minutes.
+ ~( R. C. Q, Z" N! ^置于1％硝酸银溶液在紫外光下45分钟
0 W/ U8 m' i0 P9 o2 e4 F3. Wash in distilled water. 蒸馏水漂洗1 c4 u! Q0 V/ M
4. Treat with 3% sodium thiosulphate for 5 minutes. 3％硫代硫酸钠处理5分钟
; x4 K- s9 U9 t4 X) C! G: J8 |5. Wash in water. 用水漂洗6 m/ \' O, X" c  f: k3 \
6. Counterstain with van Gieson for 5 minutes. Van Gieson复染5分钟9 y+ }, U* t5 G- f. L
7. Wash in alcohol. 乙醇漂洗
* v+ C8 l9 D0 d5 Y8 J) ~. Z8. Clear . Mount sections in DPX 透明封片

tonyyang

VON KOSSA
# f+ x- e* w  ~6 i1. Sections to water.) Y* E1 n  i$ [0 G; _4 t6 {' t
2. Place sections in 1% silver nitrate solution under ultra- violet light for 45 minutes.
/ r) \$ o5 y: M+ i3 b3. Wash in distilled water.( C2 ]+ @7 x1 M# {# s+ g. f
4. Treat with 3% sodium thiosulphate for 5 minutes.$ K4 L+ d4 n' m/ y2 @0 r
5. Wash in water.9 {. G+ u) H" ^0 y
6. Counterstain with van Gieson for 5 minutes.9 k% s; w6 n' O  [
7. Wash in alcohol.' J3 e  s: Z! f+ _
8. Clear . Mount sections in DPX ) m4 i6 |9 Q4 n% r/ V' Q9 D
4 K7 D( v% s6 p: d, G% F
--------------------------------------------------------------------------------0 E2 r+ ], A3 _0 [
RESULTS1 c: z, D/ l  X0 N# v5 a$ r8 {
Mineralised bone black Osteoid red) P/ ^. o! R/ G. m+ U; j) c4 d+ J. U
( j+ e$ J$ c7 U  i6 M# `
--------------------------------------------------------------------------------* l- w( b3 h2 K# \7 A) T, H/ t5 R
SOLUTIONS2 {( L" g0 B6 @! b3 z# x6 O: z: i
VAN GIESON --1% acid fuchsin 7.5 mls
# |5 S; G+ j( f: G: F--Saturated picric acid 92.5 mls
1 ^: \7 d$ H7 c+ N8 u--Distilled water 100 mls