求助一本书：current protocols in stem cell biology(已解决）

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Current Protocols in Stem Cell Biology( D) a. J  A' `- r# O6 w
Last Update: July 07, 20095 W  Y( j" \1 `9 ?0 N) D
Print ISSN: 1941-7322& b" L, L, G& [4 ]: ]
Online ISSN: 1938-89696 W; V% n9 x$ k- U5 a
Table of Contents
( R  p- S* X; T& r7 H9 q8 }Foreword * n* ^2 N: V$ B7 ~
Preface & U4 Y5 ~1 u; F, _5 C8 l
Chapter 1 Embryonic and Extraembryonic Stem Cells ! w1 d, b% D0 a4 [
Section A Isolation of Embryonic Stem Cells ; T/ n5 T$ g  a1 m5 k
Isolation of Embryonic Stem Cells ; o- i, j5 w2 j/ }
UNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells 2 p2 s7 f' @# x
UNIT 1A.2 Derivation of hESC from Intact Blastocysts
7 q- d  i1 }0 o) J9 PUNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells : ?. O/ V2 D, d
UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
7 t3 {0 ^- u" U Section B Characterization of Embryonic Stem Cells 8 h) \' p, \) t0 P+ _* C# u
UNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells   `, W9 m5 d4 F7 K5 D* \$ A
UNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform
" C2 \# i8 p) N+ V" j2 a& cUNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
/ y$ b. Z# s3 _" L9 R: BUNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
1 e2 H, Y) f! C0 r' c1 X$ HUNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
0 j1 ]" V& s0 | Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells 6 \% ?1 ^' [8 \3 Y8 l- J8 h
Culture and Maintenance of Undifferentiated Embryonic Stem Cells
! y3 S/ U; x; }) J0 mUNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro 9 d+ B' A# s. C" D. t+ W9 \# B3 ^
UNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture
5 m' V# ?6 x# }UNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells ; }" a- l+ |- Q8 W7 I
UNIT 1C.4 Culture of Mouse Embryonic Stem Cells 8 t  O' h& M" w) f7 P6 F; C/ _
UNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells / A9 w$ G6 P$ p9 v$ a
UNIT 1C.6 Isolation of Human Placental Fibroblasts
! V5 e# C/ [9 B2 W, PUNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells 2 d0 M3 r6 J' _3 m; v) }9 I# [
UNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor 6 V: S- f  i' G  `& T
Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
1 _) N4 P- {- VUNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap 4 J3 P5 }# Y4 I1 j8 l3 j
UNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures " C7 b0 D) E& ]! Q
UNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
) Y! I! K# s1 @: A* PUNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
5 V- P! T: |- {7 o" hUNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
6 ?3 d5 h6 o" D$ w/ E! @ Section E Extraembryonic Lineages & f2 K! ~" ]* N1 S
Isolation of Stem Cells from Extraembryonic Tissues
1 x. H* y1 p* m* B0 Z+ y3 _UNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells % L+ m& S2 y$ c; k, A
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta 5 |# o8 K$ P! ?% E+ L$ ^
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells 5 s8 B9 E( _0 ]) f$ ^# O8 Y
UNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells 3 c7 `. ]3 v5 e' H( J
Section F Mesodermal Lineages - K& ^6 d6 {# l6 B( L: E  G
UNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells . N- S+ E1 F% v0 N8 V6 O
UNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium 8 t- c+ L! e+ k9 S
UNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
" }0 d8 d# u/ s/ w/ EUNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
+ S1 P) L2 o0 O& n+ c7 qUNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells $ w) t, C. \& B( Y0 L+ P9 h$ C
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
; W1 G& L2 z  H) M2 b1 xUNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
1 z9 H) L* f3 g$ }1 ?4 }( iUNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
+ ^- `# Q9 N) `# j& a+ o6 u Section G Endodermal Lineages - N- y6 m3 A' T0 w& c# z
UNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells ) e) o6 _: q: ^+ U
UNIT 1G.2 Pancreas Differentiation of Mouse ES Cells / F6 G2 e4 Q9 o
UNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm , B) w/ d1 t. d! p- P
Section H Ectodermal Lineages ; c% W* s0 n# N! E
UNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons , M$ P% z8 Z4 A# z2 q+ ?
Chapter 2 Somatic Stem Cells
4 J8 ~9 X4 v. i3 w  Y5 j Section A Hematopoietic Stem Cells
% k. j% I9 W- D6 O3 }0 |. oHematopoietic Stem Cells 2 g7 c6 c* u  S9 V* y* Z
UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient
2 N, C& N9 \' T6 o9 xUNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
& v- }$ x( k, T. }" mUNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood * \6 K+ l/ ~% T% e: R$ n
UNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow 1 Q1 R3 C" {: a4 {2 B4 O$ W
UNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche
* L' K$ G5 `) G1 p" q9 ~: GUNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues 2 b6 U: e% s7 m
UNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells # I8 Y9 v- G0 q$ M& u) O# S
UNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells 1 q$ G5 H+ F: D
Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells
5 b9 w2 [  h1 BUNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
' p$ v: ?; U$ e9 ]8 m) t9 q2 kUNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
" H$ @4 v4 e) D3 \3 _. r; ~UNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
% T& {4 [0 M" G( T9 ]4 W% }( c! b Section C Cardiovascular Stem Cells   S2 E% Y& x: t) [
UNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
8 x) d/ x% G1 f: J% k8 u% HUNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium ! _' V1 U' S8 ^% L4 a
Section D Neural Stem Cells 2 U& ~2 o0 ?/ [9 p6 k; H
UNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains & v+ W( Y+ l: G- W
UNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells $ |2 e& g8 v. \7 z. \
UNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
- t5 a+ i+ b( y2 C# }; ^ Chapter 3 Cancer Stem Cells / y- {6 J. }7 H. R
UNIT 3.1 Colon Cancer Stem Cells
$ t% P/ k) `; m3 KUNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
1 E+ Z7 G3 I1 C% a Chapter 4 Manipulation of Potency 5 [+ m# [# M+ O3 D4 ]0 F. O
Section A iPS Cells # D8 |0 x- l8 z1 v' ?; B& A
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming * T. ]9 q4 f5 S& T% M' `1 i$ }6 ]
UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
# o1 a  ^# E2 @, O1 m! d" A Section B Nuclear Transfer & J8 F% ^4 ]! r! R- s
UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency . Z$ y# M. i6 e; J; `  z7 |
Chapter 5 Genetic Manipulation of Stem Cells
3 \  ~- _# i6 \% G( ] Section A Lineage Tracers in Stem Cells 6 F9 h) e7 D, {8 h) @* g
UNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
! z; b5 A, \4 j0 B& UUNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing
: d! O" k  h& E) G Appendix 1 Useful Information
/ V0 y# H( |/ |4 HAPPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
% g  `+ a7 x* Z% w0 xAPPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
& Y( Z( Z' F% R% u0 g4 M5 h1 n0 R Appendix 2 Laboratory Equipment 3 p1 f* x# R- v3 e5 F# a
APPENDIX 2 Standard Laboratory Equipment & {4 ^$ ~8 D" F
先谢谢啦！