求助一本书：current protocols in stem cell biology(已解决）

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Current Protocols in Stem Cell Biology
  ]1 c5 T- c/ Y+ [1 O) o7 D' A7 \Last Update: July 07, 2009
4 w9 ^$ {, A, [- K' SPrint ISSN: 1941-7322: h7 x7 l" d+ b( o, m/ w
Online ISSN: 1938-8969
0 i7 C( B2 S+ f% X' k8 _7 TTable of Contents
, o2 |& j) x; BForeword
, R5 r/ t9 o- `( \7 Q1 E, mPreface
% w1 `% S3 X! Z, R Chapter 1 Embryonic and Extraembryonic Stem Cells 4 X- R# a5 h& n7 A! ]5 E7 l& o
Section A Isolation of Embryonic Stem Cells 9 p" P5 [9 n/ t. u  W
Isolation of Embryonic Stem Cells
0 A$ f4 S2 N# nUNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
- w' a) X3 M% f- k! }UNIT 1A.2 Derivation of hESC from Intact Blastocysts
/ w6 i  |$ v3 Q& l$ xUNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells 9 U0 `+ P2 D5 X6 ^) \; P( r
UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment 7 i7 |& l* t5 S
Section B Characterization of Embryonic Stem Cells $ `/ V% Q6 j0 i) a$ ^/ j& l; k- p% [
UNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells
# W' V2 Z0 D6 t& mUNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform 2 B6 f! d( T% N0 a0 t& S: F
UNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells 0 [/ Z; [/ b9 a& `6 c2 V4 Z
UNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency 7 h2 u) J3 \" `, r. p/ }) o
UNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation : W" t+ c8 B, Y# y0 _/ L7 ~
Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
, S" }. ~5 U" g. w* CCulture and Maintenance of Undifferentiated Embryonic Stem Cells 3 \( s8 x8 h7 b; u8 s5 |6 s! {
UNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
" T5 f, c7 }' S# E! u" R" [( OUNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture % U; O9 k9 X) a7 u6 \
UNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells $ I* y: ^# x  _7 ^& L$ f, t! Z
UNIT 1C.4 Culture of Mouse Embryonic Stem Cells
2 M* b' a6 L6 p9 \: {UNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
, L) M, ]* F% }8 B$ O# U. @! a: eUNIT 1C.6 Isolation of Human Placental Fibroblasts - z6 S2 f1 }6 {# H
UNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells . k, L& V0 h% V! F9 d8 M7 g; n
UNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
+ l; g1 o; y0 G- z Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
" _7 B7 |. u; r* s- I5 QUNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap 4 }0 f. r3 r7 E& `$ P4 U8 {
UNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures 5 p6 k; O7 c7 V
UNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay $ e. g1 g0 d9 K. o- p, Q" S$ m! R
UNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions 4 i0 Y+ H+ c- l/ X3 ^7 Q
UNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells 6 p. {3 {' C! d
Section E Extraembryonic Lineages
0 }0 {, |1 y7 b# A& q" o: wIsolation of Stem Cells from Extraembryonic Tissues $ I9 r! n4 V/ [: Z
UNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells 7 z. ^( @8 o# l% j
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta 6 ?; F, ]. X4 x
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells
( `7 E5 u3 X" S7 l4 `- OUNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
- b" ?8 a8 `' F; W Section F Mesodermal Lineages 2 ?( w6 O9 y0 B
UNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells " A3 x( r. b) I2 f5 \" v# E
UNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium ( O/ Q6 q4 z6 w6 J+ h8 E/ b! W
UNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
% F4 `+ `+ d6 [& N" t  `8 x+ ^UNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
' i% {0 F. J5 VUNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells 1 y  k$ g2 {& e- H
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
- S7 @6 [1 E% r5 IUNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors ) l$ J  }4 a* u; Z6 _! m
UNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
7 i; [$ c& h/ `3 I8 g' I. K Section G Endodermal Lineages 6 P6 T8 u9 k( W: d9 j
UNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells & s- ~1 V: U4 z
UNIT 1G.2 Pancreas Differentiation of Mouse ES Cells & G! r, q; O* h7 W. U2 n% ?
UNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm 8 A1 C' o% `# \: u
Section H Ectodermal Lineages
* u  _& h$ r  i6 ZUNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons 6 I# z; D* P0 x( g; d' t+ q0 P
Chapter 2 Somatic Stem Cells 0 F2 u1 D" C' x( ~- x2 {# ]
Section A Hematopoietic Stem Cells # L# J6 n* ]/ `$ M
Hematopoietic Stem Cells 3 ?. ^; Y. q' q5 \
UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient
+ p8 W! s) e( H( ]0 `8 wUNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
$ x9 G2 d8 K/ _' g, u; t% TUNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
9 R+ B# `9 V1 D2 A' l! o% OUNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
+ b  {5 ]! D: F% j& mUNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche : [" o# [' f9 T0 v3 h# H
UNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues
6 P3 y& V8 @/ s- Q7 {; jUNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
- U% S# f- g6 V* ]7 yUNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
( r! @& I  [: T! D1 `3 }& M Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells + P9 H! [) r; ~: ^* I
UNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
* o6 q0 q. Q# P( [UNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
# e& G+ d' t% o' p/ jUNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
2 H( z5 J9 M- Y6 L, b Section C Cardiovascular Stem Cells # S# O2 N2 N5 Y3 \+ n  _
UNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
0 @! @9 }, N+ zUNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium
: i/ F# z% {! Y1 }) S9 ~! g Section D Neural Stem Cells
' [$ Z* h+ w& K- T* T8 Z0 sUNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains " x+ ^' {. a) ?
UNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells 9 P  l/ F5 C  \; N/ v  G7 a; b' w
UNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
/ @+ {, t( P1 w2 ?+ S) O/ K5 _# M Chapter 3 Cancer Stem Cells 6 ~' {; \8 t$ h4 ~0 V: C" w
UNIT 3.1 Colon Cancer Stem Cells
1 e+ F3 V) L+ q. @' FUNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model : V/ W6 V' t4 ^- w$ N1 T
Chapter 4 Manipulation of Potency $ m$ ^' x& W# }: D$ j
Section A iPS Cells   M( U, G# {7 v3 y# e
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming 4 ]- u, |, }3 o1 S* C
UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells ) I" s$ K$ v. Y$ O) c; m% ~, P# L
Section B Nuclear Transfer ) `3 U; d6 J' g, n7 A% X& U/ M: F
UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency
/ ^& Q$ M' R. c" \5 w6 J Chapter 5 Genetic Manipulation of Stem Cells
0 E4 ]! x5 ~, Z6 D! M Section A Lineage Tracers in Stem Cells
! W5 J7 U4 ~/ q' [% yUNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma + x3 j: K6 m+ D/ g" e
UNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing * ^7 ]$ Q/ |  Q& T' G" |1 H
Appendix 1 Useful Information
) h9 A; ?' H6 I0 v: t  XAPPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research 0 K4 s) y* V3 p8 H
APPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
4 i' V* L: a0 ?+ r9 } Appendix 2 Laboratory Equipment ; f+ q% q' e* e% K. J8 q6 D( v( X$ w( y
APPENDIX 2 Standard Laboratory Equipment
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