求助一本书：current protocols in stem cell biology(已解决）

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2 t: {4 {. }+ @# |0 H; K2 \0 \Current Protocols in Stem Cell Biology* ~: g: L/ U3 }" {' @
Last Update: July 07, 2009: h& W- f5 C' R2 P% [
Print ISSN: 1941-7322, p' H6 M0 H2 m; o2 L
Online ISSN: 1938-8969
, q! g5 A8 @% g' |Table of Contents
% T! ~: b( [* J- {Foreword
: f  |9 |: S' Y4 g8 ZPreface
8 a% Z, m# I( l" f2 N, ~  t9 B Chapter 1 Embryonic and Extraembryonic Stem Cells
0 J3 q8 m4 j, q Section A Isolation of Embryonic Stem Cells + ^  O8 ]: @- f
Isolation of Embryonic Stem Cells 5 l8 D. F) h. G4 C
UNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells & T2 p! I( h0 z( w8 h: k
UNIT 1A.2 Derivation of hESC from Intact Blastocysts
5 Y' w2 q6 X7 q+ j5 d0 r$ [1 y9 {8 gUNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells / Z0 ?5 m  L; b6 G- x. |# ^& Z" D
UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
% W- ]( e1 p6 A, e5 E Section B Characterization of Embryonic Stem Cells
0 U8 K* H  Y3 X9 E% r! O& tUNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells ) Q2 p5 V: R2 \2 `5 e
UNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform
! X! S" S1 G/ y, sUNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells ( R3 g4 e* a+ j* i( [. M
UNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
5 l& Z8 m0 ?3 DUNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
* H6 I  C% ?& M" J& } Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells ) E: V  t, D, P! M9 }
Culture and Maintenance of Undifferentiated Embryonic Stem Cells
& M8 ?+ c3 {: F4 r' q3 {  G" gUNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro ( r4 r' c) y: U1 E5 W$ z' k
UNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture 9 O% m. {' R2 E9 G/ [
UNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells * W4 a# M- F/ m( V- x9 {7 T* P
UNIT 1C.4 Culture of Mouse Embryonic Stem Cells
) B' f! {! O6 s% x" oUNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
- K5 s: R5 @  f$ DUNIT 1C.6 Isolation of Human Placental Fibroblasts
- p; ?) N; a: Y9 V- i3 ]& _UNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells ( A4 c9 i3 Z" Y7 c" H# R
UNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor # ]; u. l3 ]2 Z2 Y
Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
3 ?' y* G- K( x9 oUNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
; k2 Q$ P" R7 ~' AUNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
5 n5 v& r2 b# D% ~+ X4 m2 `" aUNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay $ A9 Y* \) A6 [" J7 p$ D  @
UNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions , X' w3 C' D' S; z& P0 _( E1 H
UNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells # [1 {  i6 V  ^0 l6 }
Section E Extraembryonic Lineages % P) O0 B, i+ P: F/ I& i* h- m, f& J
Isolation of Stem Cells from Extraembryonic Tissues ) |0 c* K& s+ @- v0 \
UNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells 9 D1 h# t9 c' v8 N# c/ A( g
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
3 q% e. \) |2 p5 Y( wUNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells
3 L& K! W' l: }5 N# GUNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells $ ]$ k! C  q' h9 M, D5 s; Z
Section F Mesodermal Lineages
+ h( ]' I  l% R3 C! W3 A7 y6 L  vUNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells ' K7 G+ o; J( d
UNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium / `0 _6 D1 C, f5 w1 i
UNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells 4 _( X; I% C$ I
UNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
. P! Y+ _# y# X, tUNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells
$ R, X" K6 Q. q& zUNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
! u/ L: X6 C' X5 i- PUNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors - N! {; a% P2 P6 ?; ~  }2 d
UNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells 7 [' s/ |0 s# }' t! p, z
Section G Endodermal Lineages # ^  F3 ]3 n  C+ v' I* E& R% P
UNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
2 S+ M- G) ]8 ^5 M* d- Q+ P# HUNIT 1G.2 Pancreas Differentiation of Mouse ES Cells , _/ h& ]0 K, y' \' Z; K0 y; X
UNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm 1 p* O. U, M9 f, H/ \
Section H Ectodermal Lineages # c; R( {- F3 E& c' ?* \' H
UNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons 8 F1 t$ u; N+ ]; z$ E6 a
Chapter 2 Somatic Stem Cells
$ A, r2 G8 P1 R& S Section A Hematopoietic Stem Cells
7 q; Y6 a" j6 X8 w( YHematopoietic Stem Cells 6 D' N0 E2 T. z
UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient
8 Q( C# {7 C! s+ y' tUNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
3 b: i- }) Q3 }9 {, d" |UNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood ' g" y/ e: T' t6 v
UNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
6 n2 @' H* V7 s  B5 {2 {UNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche 5 f' \5 t! Y8 P+ A
UNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues 2 j# S1 n' _" p9 W+ B
UNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells . Z4 H" ?) v( v: D" g
UNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells 7 B% [# {  v; p- [; E
Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells $ n0 B& P0 y$ |' `( J" H% ~
UNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
% l. w4 B+ n$ B' T% z1 IUNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
5 b9 b. b1 ^& j# w! L) E9 FUNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells ( `5 E" ]( W5 R, [! x2 e  p
Section C Cardiovascular Stem Cells
4 c1 z  k" e' @1 e" q# rUNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
0 e" W+ m7 V/ B- dUNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium 2 Z: O& A- r7 r- G1 |! C6 g
Section D Neural Stem Cells
- ~. L( W: S' f# r4 h, U1 {UNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains 9 g6 c. A. ]. m; {4 P9 x
UNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
1 h* q: K% \+ |UNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
! s7 c9 ~: Q8 D Chapter 3 Cancer Stem Cells
. f- `( K: F$ e7 }UNIT 3.1 Colon Cancer Stem Cells
" }; K; H2 X8 R% L" P  }* ]; kUNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model $ C+ ~8 ]4 y: o5 B
Chapter 4 Manipulation of Potency / l6 u% b) ^" z+ l6 h
Section A iPS Cells
0 a: q( Q/ L  \* L! e" C7 v$ d/ TUNIT 4A.1 Human iPS Cell Derivation/Reprogramming 6 d3 y; h6 X* }
UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
; o4 c" N; }+ @; B Section B Nuclear Transfer . n) Y0 Q* z/ d9 w7 G
UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency
  b4 |0 W) @0 ^4 u4 L Chapter 5 Genetic Manipulation of Stem Cells 6 E( R' ~9 {( Q- o! d& _3 r' L9 g
Section A Lineage Tracers in Stem Cells
) C$ ~3 m/ n6 ]. NUNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma 5 t. V; J0 J2 r7 k& }6 E- K. ~
UNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing 5 J( U  u6 u. Y
Appendix 1 Useful Information ) _) R  |- v4 \6 Z; h# R
APPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
# G) p* {2 l. Y* ]6 M1 h. XAPPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells + N0 H2 p3 X) ]: U
Appendix 2 Laboratory Equipment
+ Z7 P2 A% t0 V! \APPENDIX 2 Standard Laboratory Equipment
9 M1 {6 k% p, f6 Q5 @* w先谢谢啦！

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