求助一本书：current protocols in stem cell biology(已解决）

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Current Protocols in Stem Cell Biology% M4 n9 O0 A7 k0 J$ u; y8 i# i! P
Last Update: July 07, 2009
, D0 k( v' D  v# R& c1 HPrint ISSN: 1941-73222 p* o; H1 L" u' T$ K" P
Online ISSN: 1938-8969
0 b9 J3 i$ w# u7 |5 r' }* KTable of Contents
$ O% ^9 K, _, \( p, EForeword
( z9 _% H& B! ?  G4 e- [3 zPreface
: ?. `1 _% D; b9 V" l9 a% p Chapter 1 Embryonic and Extraembryonic Stem Cells 7 i; T6 L$ p/ I1 X1 A: S
Section A Isolation of Embryonic Stem Cells - H( w2 J  B$ Z+ M5 t8 x
Isolation of Embryonic Stem Cells
3 u7 Y/ C* b1 g% h  K% |6 aUNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells % h1 _) r) y; f& R1 }( m1 a" H
UNIT 1A.2 Derivation of hESC from Intact Blastocysts
" F1 O' r, `0 F3 _: n: u0 yUNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
# a. h; F, Z1 V6 j+ @$ I" K4 ~UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment * i7 K4 ]6 k2 y2 [  ?
Section B Characterization of Embryonic Stem Cells 3 ]5 f% g2 L2 d6 [
UNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells ' t- n! q8 h$ Q
UNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform
0 O0 [1 V) w' h8 }$ A$ EUNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
7 N; F5 N% q; y) T0 _2 i# TUNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
) K% S- B; w9 @% Y$ |UNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation 4 S9 t  a, P- v, |+ Y! d+ _
Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
) _+ }/ h' n2 \* f  L5 q8 TCulture and Maintenance of Undifferentiated Embryonic Stem Cells
. P# A: D$ G  n0 k; ^7 F; `' fUNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
  P: |* Z5 M- ]5 T/ A+ dUNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture 0 n2 q' h, `0 ^4 X/ d. D% p
UNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells , ?5 ?/ K8 r) i6 w. F
UNIT 1C.4 Culture of Mouse Embryonic Stem Cells
8 @; j8 D% Q7 g  L* BUNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells 5 _+ [: o/ e  f% k) j1 E
UNIT 1C.6 Isolation of Human Placental Fibroblasts , w- ]0 \9 k! p! ^8 G: l/ C
UNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
+ h/ d3 U4 v" dUNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor $ U8 d4 A, J9 o" J5 a! k& z( W
Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
8 Z1 |8 u& d1 O+ w: b. vUNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap 9 z/ {" Q) D% j' E; |
UNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
* H# f% O; N8 a; |& h/ H  ~* PUNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
& N- Z# i+ I4 M9 v+ S" A5 iUNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions 2 g8 ~9 W7 b- h  \: L
UNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells + F3 u# h+ ?1 E4 r  `9 e
Section E Extraembryonic Lineages
( q$ o! T; |0 \6 h( [( UIsolation of Stem Cells from Extraembryonic Tissues # B" ~' W0 A/ c+ P
UNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells ' n, R: k! Q9 G  ~
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta ) S+ T  a0 f/ N! K# y' {; h
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells
% F1 X  u- n) [8 @% \2 q3 ~; Z7 DUNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
* r' H: ]1 i" |8 Z1 x9 a3 T Section F Mesodermal Lineages ( l* P8 A& ^/ n! t; W
UNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells # B" r  U" ?( x8 [. ~; B) q; Z
UNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium
/ f& {' ?3 Z1 @# q( aUNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells , V5 {5 r1 Z; F, f
UNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
/ B) h$ `/ Z# kUNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells 9 r9 E5 b2 h& l0 D3 N  B' m
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
! ]- y. ^0 ?& JUNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
9 `9 P9 n5 h' h: z% b8 |) UUNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells 8 ?# K9 {' W" {# S( j9 m1 p0 e1 U# d
Section G Endodermal Lineages
, C- W+ J% w) {/ d  Q$ [2 Y% _. YUNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
4 l0 r2 F+ d* X; B! A) y( {2 |UNIT 1G.2 Pancreas Differentiation of Mouse ES Cells
7 X* U. a1 I9 N( m1 x- I7 h9 C& vUNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
: e  L5 |3 L' T( N7 J( I0 N Section H Ectodermal Lineages 0 A# T: l5 r6 B8 L9 u1 D, m
UNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons : H& ~3 n8 z% P5 B; ^
Chapter 2 Somatic Stem Cells + S- z3 `7 o' c
Section A Hematopoietic Stem Cells
/ b+ G; Y6 q% X8 W0 C/ d7 aHematopoietic Stem Cells , B3 ^" Y! _6 {4 e; }$ V
UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient 1 O2 `% {& G. V8 H# \5 x
UNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood 5 X+ @. O$ Y( B7 X- C4 h
UNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood   X+ g, y0 q3 Y% _# F
UNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
& t: I( h8 l% x5 V1 FUNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche
; k, ]8 ?" l+ N0 wUNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues / ~2 W, `* t0 r: z3 C+ ^
UNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells 7 M7 B" b" l" @! [! _( n
UNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
& v) K! k/ a3 x/ o. u8 \5 d! W/ q6 Z Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells
7 H6 a: N0 |0 x' HUNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
3 V. y+ Q8 b' {# o% Q' t5 B8 NUNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells 8 c4 i0 j/ o6 G, }3 `: K6 }( e
UNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
# n: \5 G5 x/ B- b. y, f Section C Cardiovascular Stem Cells
0 y/ H* Y$ a* x2 e2 D! ^; B2 B2 CUNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood ' m4 l% ]; a" U: }
UNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium $ {, h. i9 a& n
Section D Neural Stem Cells
2 m- t, \- Q1 Z% t! gUNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
) \3 [1 }3 _2 ^% C& d& v0 }' X) u' }# Q1 yUNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells . Z# F, N: Z$ T% c1 U3 ^
UNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
' P" P# ~5 m" t& w Chapter 3 Cancer Stem Cells + Q) b1 q. Q9 b
UNIT 3.1 Colon Cancer Stem Cells
) K3 Y) `6 Y0 l' C# @! gUNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model 8 F; K6 w' f% ]6 ~2 k9 v2 c
Chapter 4 Manipulation of Potency
$ o! m; M1 R, z, Y Section A iPS Cells 9 o8 @" t4 [+ a( @, q. j
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming
1 E" n8 s2 [9 i. M  S! [, ^- P8 ZUNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
. n4 Q' M! M0 e& m1 b8 G Section B Nuclear Transfer # s! q" _1 g: d6 c. Y: E
UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency % n/ L/ [6 g; b) h
Chapter 5 Genetic Manipulation of Stem Cells + i( C# B$ P  m$ A8 v- b
Section A Lineage Tracers in Stem Cells
: b; Z* V7 w5 M9 z/ o( F' AUNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma : Z$ d2 G" }& F7 B
UNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing
. x9 `& @' D' ~( R7 h3 h Appendix 1 Useful Information - a! p& Z- N, V* O+ D2 ]
APPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research 1 c) B# M: e& F: K5 g4 I
APPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells 6 `& l* l4 A3 ^$ @" Q* N
Appendix 2 Laboratory Equipment 2 [$ X0 a( t6 A
APPENDIX 2 Standard Laboratory Equipment
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