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本帖最后由 细胞海洋 于 2009-8-13 12:01 编辑
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Current Protocols in Stem Cell Biology
& A- M O6 s q NLast Update: July 07, 2009
" h- h$ V! x6 `/ f6 o( \( FPrint ISSN: 1941-73220 S3 m, z ]0 [/ }) ]
Online ISSN: 1938-8969
& f$ B- t1 O' M* X# XTable of Contents
" d7 \ s. w. B9 G- jForeword : y# X/ x. |4 D! c3 g. M# | k" c
Preface : u7 f% z- F2 A! z. z; q
Chapter 1 Embryonic and Extraembryonic Stem Cells
$ Q+ l& r& z3 }2 T/ H Section A Isolation of Embryonic Stem Cells 3 r+ e, l/ Y" Z0 j* P; U" e
Isolation of Embryonic Stem Cells
) U( m* ?( S: |2 _UNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells * V" e' j1 V6 a' q* A
UNIT 1A.2 Derivation of hESC from Intact Blastocysts
$ Q! r8 ?% |: KUNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
1 u9 v6 [1 J$ P9 _/ aUNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment 2 T5 M# g' ?6 t5 I& ^9 r. p: D( R. f
Section B Characterization of Embryonic Stem Cells " R) B; Y0 t9 `' u0 Q3 g# Q
UNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells
2 `+ X, \4 q2 V5 I& B n8 rUNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform
3 Y3 m7 A% @3 C7 IUNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells # r1 a8 Q5 R: l; k8 A
UNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency " Q l- g2 l6 ^& k ~& z2 k
UNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
5 ]3 T9 \8 }& b9 X) P* F Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells % H' ~8 m3 V9 _3 G
Culture and Maintenance of Undifferentiated Embryonic Stem Cells , Z4 f( M0 _' E, M/ n! G2 P
UNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
5 X5 U8 h! e% CUNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture
7 Q: {$ y7 p! ]- ZUNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
0 w* r' C K# r! D$ sUNIT 1C.4 Culture of Mouse Embryonic Stem Cells
# {* R3 I5 i; D7 d* k: n7 \, LUNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
( W L& n* ^ j3 H" aUNIT 1C.6 Isolation of Human Placental Fibroblasts / U" L2 ~, O0 \3 W+ i/ D& ^
UNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells , p p b* K( V, d$ D1 r2 t0 M
UNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor : y5 B1 M# i6 z
Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells * n2 G9 a6 j* N5 m
UNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
& {+ C8 I ~" m! ^3 l M3 D' v' LUNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
% E9 R: `! H' o7 c9 G* vUNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
0 I* G3 z/ {* x. VUNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
, ^* r% z! H4 d q/ f0 NUNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells : A1 B, _6 h% T2 O( L
Section E Extraembryonic Lineages
3 i# E2 _( w; `Isolation of Stem Cells from Extraembryonic Tissues
0 P6 h9 O5 a& c4 v3 e/ n3 kUNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells
3 V, T* Z* O: b2 w9 |2 |2 ?- A* IUNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta & U9 \" o8 c$ r
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells
: G X% Q3 ~) l! k. Q- ~/ w0 W( pUNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
! b4 p! P# d+ r. ]4 ~; O" N Section F Mesodermal Lineages
; i& E8 d9 ~' A5 u2 r. @2 \; MUNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
3 J: [: y6 i d7 Y! A1 [8 \UNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium % a$ O6 u7 r: v" ?9 `% Z
UNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
/ C* h8 m0 k0 o4 t4 a! TUNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
5 q4 |4 r4 B6 J9 J* e5 [UNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells 1 \2 s* K! |; |" x8 G( T/ z
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers : f, ?4 @5 V7 v: B( j% C
UNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors 6 A& _: _2 a* H+ g( n, x
UNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
. V5 F* A ? ]0 s- m0 `% S Section G Endodermal Lineages
, B2 D% @5 I3 @, \/ a9 P8 G9 sUNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells - |9 X: l# |% Y+ B+ V( I3 y% B
UNIT 1G.2 Pancreas Differentiation of Mouse ES Cells , K, Y3 k& e, v
UNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
' G( V* ^3 N8 K V' Y7 Q Section H Ectodermal Lineages
: y% F, S) W A4 @8 [4 tUNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons " L9 b( h& H( V6 Q: w
Chapter 2 Somatic Stem Cells Z: w, g) k- l. F. p7 M
Section A Hematopoietic Stem Cells ( h, C' S5 ~: c& o: V0 j7 ?
Hematopoietic Stem Cells : [* A# W9 @& V; s- P8 H! k
UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient , } ^8 X9 u5 ^" z$ v
UNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood # u( o& v; D0 ], K/ ~- d9 [
UNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
( x- X4 t3 L* PUNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
( ^: V/ z; g0 F( w* h1 U; SUNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche
+ Y: {8 o/ e1 GUNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues $ K" f* L. l, y$ o) i8 J
UNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
! \8 J* n8 X( BUNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells ; J# U: l8 Q p9 }
Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells
$ o. Y& p9 Z" L. S' f# x8 BUNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
# | B3 f1 R" l. f& f. B/ {4 RUNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
N9 M; Z/ H( a9 e, FUNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
% D: X, n) e1 A/ z- d! Q& g" M' B3 M Section C Cardiovascular Stem Cells ) v3 t- ~; }! y" Y0 [0 h, v
UNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood 7 @( A9 o7 [5 Z$ n! G5 Z! r
UNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium
( p/ g+ K% e8 M0 v! I* q- D Section D Neural Stem Cells
, U e9 C7 A8 Q X) lUNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
+ z. P4 I$ K; ]8 o5 X7 YUNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells y3 F" ~, m$ s( m6 E V* [
UNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
! G& }% h7 u7 m4 A: v- G5 Y3 G Chapter 3 Cancer Stem Cells # |) [( b/ j* T8 Q# A! a* k
UNIT 3.1 Colon Cancer Stem Cells
9 h" y" m9 y, B0 T9 nUNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model 6 {1 t) [0 y* a) Y
Chapter 4 Manipulation of Potency
* m* W* d" w. X0 L* Q* }8 ^ Section A iPS Cells / Q/ M/ j* g' ?# |
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming 0 S5 q2 x4 ]; R3 K7 W
UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
$ x! w0 C) v2 K; q c Section B Nuclear Transfer 8 ]! u% x0 S' e% S
UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency
6 r6 d7 ?3 b; z Chapter 5 Genetic Manipulation of Stem Cells
+ I& N; M) a, ~6 e) J$ S Section A Lineage Tracers in Stem Cells
* r: c E, J% G4 hUNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
5 U8 Q% }: ^5 e3 { z' gUNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing
1 ~5 A, H4 ^) ? x: U, K Appendix 1 Useful Information
. H. d/ g! j3 f. [, WAPPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research + X5 ~" ^( T+ P! C4 N9 i9 ^0 U
APPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells & n5 _4 C4 M8 q5 k
Appendix 2 Laboratory Equipment * P" Y- _. ]( d l" |, E" n& _7 w
APPENDIX 2 Standard Laboratory Equipment 3 S, ^' Z7 T% u$ u2 h* k
先谢谢啦！ |
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发表于 2009-7-28 16:42
发表于 2009-7-29 16:23
