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1: Cancer Sci. 2009 Aug;100(8):1397-402. Epub 2009 May 6. : L+ k, w* |( s6 N) a7 [9 j' p
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Cancer stem cell-like SP cells have a high adhesion ability to the peritoneum in gastric carcinoma.
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Nishii T, Yashiro M, Shinto O, Sawada T, Ohira M, Hirakawa K.
' S+ I* P/ r8 q# l! HDepartment of Surgical Oncology, Osaka City University Graduate School of Medicine, Abeno-ku, Osaka, Japan.0 A9 T2 t  K* j7 y3 I( g0 q7 V8 K
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Cancer stem cells (CSCs) are considered to be responsible for cancer metastasis, but the evidence to conclusively prove this hypothesis remains uncertain. The side population (SP), as evaluated by a flow cytometric analysis using Hoechst 33342, has been known as CSC-rich population. The aim of this study was to clarify the characterization of the SP cells in peritoneal metastasis of gastric carcinoma. Gastric cancer cell lines OCUM-2M, OCUM-2D, and OCUM-2MD3 (a daughter cell line with high potential for peritoneal metastasis) were used. We isolated SP cells from OCUM-2M and OCUM-2D using flow cytometry. Serial sorting was performed three times to enrich SP cells, and they were designated as OCUM-2M/SP and OCUM-2D/SP cells. Flow cytometric analysis showed 0.46%, 0.29%, 5.24%, 6.49%, and 11.3% of the SP cells to be found in OCUM-2M, OCUM-2D, OCUM-2MD3, OCUM-2M/SP, and OCUM-2D/SP cells, respectively. The intraperitoneal inoculation of SP cells and OCUM-2MD3 cells produced peritoneal metastasis, but parent cells did not. The adhesion ability of SP and OCUM-2MD3 cells was significantly high in comparison to that of parent cells. The expression level of adhesion molecules alpha2-, alpha5-, beta3-, and beta5-integrin, and CD44, was high in SP cells compared to parent cells. The expression of stemness markers, Oct3/4 and Sox2, increased in the SP-cell-injected tumors. These findings suggested that CSC-like SP cells expressing alpha2-, alpha5-, beta3-, and beta5-integrin, and CD44, may play an important role for peritoneal metastasis in gastric carcinoma. Oct3/4 and Sox2 may be associated with CSC in gastric cancer.9 b, G6 |9 Y" G$ M
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PMID: 19493275 & t1 @) m2 r8 ^3 q; `
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pubmed链接：http://www.ncbi.nlm.nih.gov/pubm ... nel.Pubmed_RVDocSum

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