READING TIME TOPIC 24

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% g% N/ H+ U) A! T* ^Today' s topic: Lentiviral vectors: excellent tools for experimental gene transfer and promising candidates for gene therapy
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$ M$ O- f) C/ @' s+ ~8 SThe summary and the paper is in the attachments.
' Y; Q- X0 G% s1 you could try to translate the summary* ~2 p" V& \* t6 ?
2 you could talk about the author or the institute% @$ R3 o; E2 m7 x6 ], M' F0 y; |4 }
3 you could summarize steps of the experiment or the results' ~6 }1 y/ p+ g! |2 H" ?
4 you could give your doubts of this paper
0 |5 Z' ]/ ^; a. \1 m7 |) \- x5 you could upload your record of the summarize
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' X- b  `  D; _; gIf you want to talk something else, please let me know.

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Luigi Naldini
* a4 l! Y- Y- WGene Transfer Technologies and new Gene Therapy Strategies, e0 C9 f+ a/ m. Z7 y. F9 o
luigi.naldini@hsr.it
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. g9 n% ?; b) M6 DSomatic gene therapy requires the efficient delivery and sustained expression of therapeutic genes into the tissues of a human body. Such an approach has tremendous therapeutic potential for several inherited and acquired diseases. However, several challenges must be met to fulfill these expectations, starting from the development of more efficient, safe and versatile gene transfer tools.

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introduction9 j8 |5 E# e2 `( x7 o
design of lentiviral vectors
# Z- \8 l1 i2 A& j# N) t" ktransgen expression by lentiviral vectors( [: K3 ]+ B  b9 _: V' g6 G  x1 d
biosafty of lentiviral vectors6 S8 Y* o0 X5 [5 P* M
non-primate lentiviral vectors7 \5 P5 O0 Q- }9 A' b8 D
gene transfer performance
  w  V& a8 r! n  @5 j+ v. pconclusions and perspectives
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introduction
9 F) t3 F4 o# F/ zdesign of lentiviral vectors
  `3 X: N2 g0 H/ C7 Dtransgen expression by lentiviral vectors
" c. m; y+ c3 f+ U$ }7 _+ Ybiosafty of lentiviral vectors$ x9 _5 s& ^( O* N7 l1 c# n
non-primate lentiviral vectors( n* m+ _' K" q6 v
gene transfer performance+ @4 x- B! U' U2 `& S
conclusions and perspectives& K+ }8 E( h# ~6 e/ `
reference

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摘要, k7 n3 d5 Q* \1 J" t2 {, Z# t
来自于慢病毒的慢病毒载体是转基因实验的工具。慢病毒载体从首次应用到现在，已经在设计，生物安全，在目的细胞中转基因表达能力上有了很大改进。目前可用的灵长类和非灵长类慢病毒载体，应用这两种系统在大量组织中的表达的研究在不断向前推进。这里我们回顾在基因治疗方面潜力的慢病毒体系。

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QUSETION:
0 w: _% c- n& e" |1 What' s the differences between primate lentiviral vector and nonprimate  lentiviral vector?what are they?" V$ h9 q* f- g5 A+ j# j. C
2 shall we use lentiviral vector in vivo?

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please join in actively, you can learn a lot.

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"HIV can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa, while HIV-2 viruses are related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Group M can be further subdivided into subtypes based on genetic sequence data. Some of the subtypes are known to be more virulent or are resistant to different medications. Likewise, HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses, although HIV-2 is known to cause AIDS."
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& B+ W. B% g( c0 s' c; c# eso primate and nonprimate lentiviral vector are different origins. they used for different hosts.