Induction of iPS

刘流

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
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, `, n+ o# b+ [9 R* b2 PKazutoshi Takahashi,1 Koji Tanabe,1 Mari Ohnuki,1 Megumi Narita,1,2 Tomoko Ichisaka,1,2 Kiichiro Tomoda,3 and Shinya Yamanaka1,2,3,4,*
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* u: a9 S" R( w# g$ x3 dSuccessful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate thegeneration of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells weresimilar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiateinto cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult dermal fibroblasts without MYC.

鸽儿

old and short ! better learn ！

托斯卡纳教父

这文章有些年份了吧

juan880929

都是好文章

yzc820215

这的确是很老的文章，但是经典啊。