PDF电子书：Current Protocols in Stem Cell Biology 2007

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) g6 D' V  k% ^! [3 d) ~: fFOREWORD
: }7 h6 |, [  F, ]  vStem cell biology is emerging as a field in biology with tremendous therapeutic potential.( o) @. s9 u9 m( \( P- E1 B
Making this potential a reality requires an international effort. The recognition
3 ~" W) `, D: h) z( Pthat such a promising yet multifaceted discipline needs fostering led to the establishment
+ L: N* N8 x2 x: s4 N3 Eof the International Society for Stem Cell Research (ISSCR). Central to the efforts of the
' u5 e5 Y: ?0 S) {ISSCR is the development of tools to ensure the success of stem cell researchers. What& G2 N1 d9 @; y
better way to do this than to collaborate with Current Protocols to develop this valuable
) J9 S7 S3 H% ~, S) A4 @compendium of protocols in stem cell biology?+ Z( R1 _6 Z5 K
Stem cell researchers have developed a number of breakthrough techniques, from the
. H" C( H2 f& Q. ~. y& bderivation and manipulation of pluripotent embryonic stem cells to purification and' H( s7 j7 f2 E! ~. v7 e3 o
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories9 l9 _. A8 c& ?1 T, H0 D* b
have become leaders in the field as a result of developing such techniques. The more6 \1 ~' J, j0 y' }# d
efficient scientists are at implementing new and powerful methodologies in their own
; l# c9 r! F) ~% Y7 d$ H/ tlaboratories, the faster stem cell biology will advance our understanding of normal& q- s) m3 p( |# g# X
development and lead to the development of therapies. Thus, the availability of quality: Z: J) a0 ?) a
protocols will have amajor impact on the success of the entire field. Current Protocols has
7 d8 p" h3 J! \1 B0 @' k! s& glong been the premier volume for proven in-depth protocols regarding many aspects of. q6 g" H( s% Q
biology, and this volume on stem cell biology will prove a valuable addition to researchers
# H6 c5 p9 b4 ~! {3 u. ]worldwide.; [1 J$ _1 C# ^( ~
Experiments in stem cell biology must be interpreted with great caution as well as
* l3 H9 H+ e8 Topenness to alternative explanations. For example, the recently discovered phenomenon  M# o( [, H4 {  @
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
) p- g+ O+ u* f8 stissues were initially misinterpreted as evidence for stem cell trans-differentiation.# C" C) n* r0 W3 U' F/ o, b
It is very important that this compendium of protocols highlight potential pitfalls as well
2 h! r# I6 n, z1 U# Y! t$ ]( _as maintain the opportunity for clarification and correction when the need arises. The
' j4 {; U% f* c3 V; kfact that these protocols will be provided online will help ensure that researchers always
* T3 Q; `+ K$ T+ fhave the latest, most up-to-date protocols available to them.# X% V6 j, F3 {* m  g5 }
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine
. x& _- o0 O; S7 J8 @& ^4 X3 d! t/ {push to share information and interact so that the field can move forward quickly. There
* W! {* N" r' `3 J: dis a drive to develop not only excellent basic research skills but to bring the findings8 f% m$ L% i5 e1 d% B6 D
to clinical use so that patients can benefit. As a Hematology Attending Physician at( [7 T4 l( q: c; H2 l1 {% R
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia2 a, ~/ b$ ?" |
and am drawn by the need to translate our research findings into therapies to help treat a% w2 U# B3 z; V7 {0 L7 E8 d
number of diseases. The promise is great, but we need to deliver, and I believe Current
  i. ]) w2 D7 nProtocols in Stem Cell Biology will help tremendously.+ ]* N3 u  l' V  X+ G
Leonard I. Zon
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