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本帖最后由 细胞海洋 于 2010-1-25 00:13 编辑 " t% s) o3 D6 s# G# U
* e+ T! R" z0 [1 sFOREWORD
& _: P) M' c6 m/ D- w2 x* BStem cell biology is emerging as a field in biology with tremendous therapeutic potential.+ J4 F6 P" D0 Z: ?
Making this potential a reality requires an international effort. The recognition
* z9 l/ W) u# Z3 o9 ^that such a promising yet multifaceted discipline needs fostering led to the establishment
. |3 v! g+ P( |4 T5 o- Lof the International Society for Stem Cell Research (ISSCR). Central to the efforts of the) ^0 A4 f$ Z* r7 X5 E9 Q
ISSCR is the development of tools to ensure the success of stem cell researchers. What5 s* y. V3 e- r2 k u7 [
better way to do this than to collaborate with Current Protocols to develop this valuable. p, P. V0 }5 r
compendium of protocols in stem cell biology?' k! z$ ?2 |. y( L
Stem cell researchers have developed a number of breakthrough techniques, from the
5 A8 }+ |& d& o* U U5 H0 Vderivation and manipulation of pluripotent embryonic stem cells to purification and/ ^/ e- A) Y! h+ V0 |( D2 l
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories5 ?+ q) R9 ^8 L0 G) E
have become leaders in the field as a result of developing such techniques. The more
3 r7 R! K, @+ a8 R5 Iefficient scientists are at implementing new and powerful methodologies in their own7 k9 E5 w2 {0 B
laboratories, the faster stem cell biology will advance our understanding of normal
* _0 a( D0 g+ j5 @- M7 V7 ^" N. \development and lead to the development of therapies. Thus, the availability of quality, r% F3 s0 k( j) T
protocols will have amajor impact on the success of the entire field. Current Protocols has
$ P l/ w$ \& V# u1 blong been the premier volume for proven in-depth protocols regarding many aspects of" Y% ]2 \( p* K5 q: X
biology, and this volume on stem cell biology will prove a valuable addition to researchers
- U1 X; l1 z" _/ X" tworldwide.. {7 {. a e8 X
Experiments in stem cell biology must be interpreted with great caution as well as" T' ~2 I" U! p
openness to alternative explanations. For example, the recently discovered phenomenon# }" S" r1 U- W" V
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral8 K" {& x4 ]2 G$ U/ B0 E
tissues were initially misinterpreted as evidence for stem cell trans-differentiation.# x( F* _+ b7 K; R
It is very important that this compendium of protocols highlight potential pitfalls as well
X: Y. L& G, Q& D3 i- v8 F7 Was maintain the opportunity for clarification and correction when the need arises. The
: L. O1 a" A" y* }, vfact that these protocols will be provided online will help ensure that researchers always* v* F3 A! L& P9 }1 S( C
have the latest, most up-to-date protocols available to them.& {6 V/ g: I' ?. e, J
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine5 y& B+ k! _5 m8 d9 Q. V
push to share information and interact so that the field can move forward quickly. There: R* A; i/ {* B5 @) b- ]
is a drive to develop not only excellent basic research skills but to bring the findings1 r- H4 @& K3 ? e/ @% D0 j
to clinical use so that patients can benefit. As a Hematology Attending Physician at9 k0 @; a7 c$ L* t' F/ M" z
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia
9 P- b2 S* B# L: c" [' pand am drawn by the need to translate our research findings into therapies to help treat a0 x( Z3 Z! e P" u# C
number of diseases. The promise is great, but we need to deliver, and I believe Current
0 {0 S8 V2 a5 s# ^: H4 b7 i) nProtocols in Stem Cell Biology will help tremendously.7 O& H, f: S; |; r% b5 d/ w
Leonard I. Zon1 F. \2 \; Z8 W( a
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