PDF电子书：Current Protocols in Stem Cell Biology 2007

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FOREWORD) x; A% m; D' \1 b) O9 w  C
Stem cell biology is emerging as a field in biology with tremendous therapeutic potential./ D% J1 r0 K, ^. \" Q* y
Making this potential a reality requires an international effort. The recognition
! ^0 Y0 w7 O. p1 Z1 ]8 b5 _that such a promising yet multifaceted discipline needs fostering led to the establishment  a. K  D. S3 ]# s. `7 t7 w/ j4 E
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the
& q) K) T  w, Y2 d% D& q( rISSCR is the development of tools to ensure the success of stem cell researchers. What
0 G& G' R6 o' Y+ b$ k$ cbetter way to do this than to collaborate with Current Protocols to develop this valuable2 ^7 s( w9 }% Y/ U' z9 C$ u& ~) t
compendium of protocols in stem cell biology?
8 Y4 `3 J: o# ]% ~' KStem cell researchers have developed a number of breakthrough techniques, from the1 V2 u0 ^  ]0 o' U
derivation and manipulation of pluripotent embryonic stem cells to purification and" \4 K+ D( y  E
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories2 X; l; J/ J7 V  m
have become leaders in the field as a result of developing such techniques. The more- A- q1 O4 R. P% x; g/ F$ G
efficient scientists are at implementing new and powerful methodologies in their own
5 g: D8 g% B' ?5 y  y. Claboratories, the faster stem cell biology will advance our understanding of normal
9 U' K  \8 `" ~5 m# hdevelopment and lead to the development of therapies. Thus, the availability of quality3 N6 T; r; J! t3 O7 F
protocols will have amajor impact on the success of the entire field. Current Protocols has8 A7 _! [& f/ H
long been the premier volume for proven in-depth protocols regarding many aspects of* }9 l9 e; X" q6 }7 Q& I
biology, and this volume on stem cell biology will prove a valuable addition to researchers
% m( z$ o. O+ S4 d3 Pworldwide.( n  F$ l3 D6 q% n1 ]- E( H: q) O  ~
Experiments in stem cell biology must be interpreted with great caution as well as3 V' C8 I  _- Q
openness to alternative explanations. For example, the recently discovered phenomenon
3 x! e1 U! u. s. Oof cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
8 B& m# |" q4 k; W; x0 p* ?/ P9 a% U2 w+ Utissues were initially misinterpreted as evidence for stem cell trans-differentiation.( z; K) V% p; d3 s" V, L
It is very important that this compendium of protocols highlight potential pitfalls as well+ Y7 b( K& s5 ]- g7 m% Q
as maintain the opportunity for clarification and correction when the need arises. The/ V& n+ R( m* d
fact that these protocols will be provided online will help ensure that researchers always
- f9 L. t  f5 C* h3 Mhave the latest, most up-to-date protocols available to them.  l7 f8 V+ h* v( W# O
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine! C- o$ h- Y/ V$ v9 ]! S# s! v
push to share information and interact so that the field can move forward quickly. There
( ?  _# y9 J1 A6 s: xis a drive to develop not only excellent basic research skills but to bring the findings
$ k8 v! P9 r" X  Zto clinical use so that patients can benefit. As a Hematology Attending Physician at
5 A% v! Z0 t: `1 m' Z) c5 AChildren’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia3 s7 @8 k. u( v
and am drawn by the need to translate our research findings into therapies to help treat a$ n: [# @9 ?! v8 ?
number of diseases. The promise is great, but we need to deliver, and I believe Current2 g2 P; ]5 U/ f* Z6 @$ u" z( F
Protocols in Stem Cell Biology will help tremendously.7 s# V. B  y* v. X
Leonard I. Zon  b; c* {3 B% U: O0 J
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