常用的β-actin 引物序列

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human actin f ctc cat cct ggc ctc gct gt
% s# x0 e. @+ ehuman actin r gct gtc acc ttc acc gtt cc # W: p/ ~3 ]/ J6 H
product size:268
5 l+ `: l0 P: B" d/ L' d; zrabbit actin r agt gcg acg tgg aca tcc g
* |1 }' o# q" F# Z6 ^rabbit actin f tgg ctc taa cag tcc gcc tag 5 M0 d& z' w3 U/ b1 v7 g- K
product size:295 # u. N% x* k+ i
mouse actin r cgt tga cat ccg taa aga cc
3 g: f% N5 i7 |5 R6 q' [7 omouse actin f aac agt ccg cct aga agc ac # x: R2 |% T# |+ A
product size:281
# J* L0 K2 n6 I9 M* z! D' K3 [rat actin f TCAGGTCATCACTATCGGCAAT
7 Y2 _% g0 W- [$ J6 M! X! [; yrat actin r AAAGAAAGGGTGTAAAACGCA
6 V6 v7 K9 C  A% i, fproduct size:432
! Q4 T8 A/ J: P6 X% T, {1 phuman actin r gag cta cga gct gcc tga cg
/ l) G9 I+ Z! Hhuman actin f cct aga agc att tgc ggt gg
$ _' x3 T; x( }$ nproduct size:416 6 A% R! u; |( z% ]
mouse actin f tca tca cta ttg gca acg agc 4 i. h0 K) m: }+ Q: e( r0 h
mouse actin r aac agt ccg cct aga agc ac
( t% m+ z- ?- ]/ k& y$ T: Mproduct size:399
2 l" }& G2 j$ L. m7 j4 `rat actin f CCCATCTATGAGGGTTACGC   f5 P+ M0 _& `( [! f# R
rat actin r TTTAATGTCACGCACGATTTC
. V5 t: L. f+ i' Pproduct size:150 ! k, W+ u$ M( a5 F
rabbit actin f tct tcc agc cct cct tcc tg 7 K: U( G0 U) D
rabbit actin r cgt ttc tgc gcc gtt agg t ! s' W5 \* [. F) ^
product size:409
& p  G3 A# h5 Y9 A% }内参基因名称 引物 引物最佳退火 扩增
" D) M+ }# |+ z. ~* }* ]) S基因库序列号 引物名称 序列 位置 Tm 温度C 长度 ; q2 [- i; t$ l/ x- ^! Z5 R6 R
3 U  L4 F" `3 @3 ~
Human actin beta F305 ctgggacgacatggagaaaa 305-324 52.3 + a, R6 b7 n0 T7 z, A
BC002409 R868 aaggaaggctggaagagtgc 868-849 52.6 59.4 564 ! X; }# k& {  V( Q* T8 x; V0 g5 s
F1379 agcgagcatcccccaaagtt 1379-1398 57.3
/ @  c/ G' n" d6 w8 C& ?" fR1663 gggcacgaaggctcatcatt 1663-1644 56.3 54 285
4 ~1 L! B# M9 _$ v  Z% @& a$ F5 d3 j" o5 o( w: ]) ?7 {/ ^
Rat actin beta F18 cacccgcgagtacaaccttc 18-37 54.5
- w2 m. U( G) y: KNM_031144 R224 cccatacccaccatcacacc 224-205 54.4 60.4 207 % w% v" r% f* }
F694 gagagggaaatcgtgcgtgac 694-714 54 ) b9 _, G% L" A2 c4 N9 Z
R1146 catctgctggaaggtggaca 1146-1127 53.2 57.1 452 0 I: V$ v0 X& w3 e3 x) w! ?6 W  x4 O
! N. |. m3 ]: B# o& |2 [1 {
Mouse actin beta F91 atatcgctgcgctggtcgtc 91-110 57.5 9 c$ b& H1 B1 W  X9 o" d
NM_007393 R607 aggatggcgtgagggagagc 607-588 57.8 60.4 517
3 R7 j" c4 {! C4 w# o! kF1566 gtccctcaccctcccaaaag 1566-1585 54.5
$ u' X, E# n/ H5 \0 iF1831 gctgcctcaacacctcaaccc 1831-1811 54.4 55.7 266 7 y; P4 g6 v9 p4 }& n8 g1 e& K
# L& L3 V$ N# `
human GAPDH F369 agaaggctggggctcatttg 369-388 55.6 1 G2 k' @$ [' x+ a3 c0 F
BC004109 R626 aggggccatccacagtcttc 626-607 55.1 57.5 258 8 u9 t& ^5 f6 v& G2 X! \
F66 aggtcggagtcaacggatttg 66-86 52.5 ! v$ {7 t- {. }" f
R597 gtgatggcatggactgtggt 597-578 52.6 57 532 . V1 b8 @+ {7 I% D( f0 ?' h

- S  [' d& k+ [; ]Rat GAPDH F50 cagtgccagcctcgtctcat 50-69 55
8 Q) F. m+ u2 p# K0 M: hBC059110 R644 aggggccatccacagtcttc 644-625 55.1 58.3 595 4 ]* {# d6 \. P' v( |( `; ?
F1029 acagcaacagggtggtggac 1029-1048 54.4
) x7 i& B5 t" L7 @- v( O  K# k  {R1280 tttgagggtgcagcgaactt 1280-1261 54.8 57.7 252 9 m' l2 c( F. y  _# Z
. p" M& {2 R! c9 ]) y0 X
Mouse GAPDH F306 aggccggtgctgagtatgtc 306-325 54 ! l6 j4 Q% L4 k( {0 [3 `
M32599 R835 tgcctgcttcaccaccttct 835-816 54.6 58.7 530
! e: o$ J9 I$ X3 AF49 ggtgaaggtcggtgtgaacg 49-68 55.1 * |% I; n) m  S# }! |$ F
R281 ctcgctcctggaagatggtg 281-262 54.5 56.8 233 + |  M1 F9 P9 D: G9 t2 m

- r/ {7 q5 ?* LRat 18S rRNA F1865 tgcggaaggatcattaacgga 1865-1886 58.6
, O% y: L/ Y% }5 c0 ^- ~6 C6 WM11188 R2164 agtaggagaggagcgagcgacc 2164-2143 59 59.3 300
9 ]/ ^( d; s% ~" u8 d) Y
& z: X* U" C9 t8 j6 t7 nhuman 18S rRNA F1565 cagccacccgagattgagca 1565-1584 58.3 ( R8 v) H1 J# I5 D4 S
M10098 R1816 tagtagcgacgggcggtgtg 1816-1797 58.3 59 253
! c+ ]$ V4 o$ f- o! s' q, m3 s
6 u5 K% N6 j" Nmouse 18S rRNA F56 aggggagagcgggtaagaga 56-75 54.4 $ t  O# E; K/ l! T- U
K013364 R296 ggacaggactaggcggaaca 296-277 54 62 241