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(1)http://www.nature.com/nprot/journal/v5/n3/pdf/nprot.2009.236.pdf/ w' ~0 H7 y$ l1 Z0 ]# w
Nature Protocols 5, 516–535 (1 March 2010) | doi:10.1038/nprot.2009.236 ! A( z2 ?( s, D, }/ c8 t" |* w
RNA-Seq analysis to capture the transcriptome landscape of a single cell.
1 u2 r$ X5 b- {, J( \(2)http://www.nature.com/nprot/journal/v4/n11/pdf/nprot.2009.119.pdf
5 e3 N/ i- K% b. GA reporter gene imaging system for monitoring microRNA biogenesis .* ?* l8 O( g2 n2 p) S; Q; j- z
Nature Protocols 4, 1663–1669 (1 October 2009) | doi:10.1038/nprot.2009.119
+ t" k# k& |5 D5 u(3)ttp://www.nature.com/nprot/journal/v4/n9test/pdf/nprot.2009.145.pdf
, V: }" }+ B( b7 q1 K. Z% h/ n; H! ]Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression .
- u6 H7 a" c8 ^0 x6 |: UNature Protocols 4, 1338–1348 (1 August 2009) | doi:10.1038/nprot.2009.145 * E) m4 D5 o2 p. O- F
(4)http://www.nature.com/nprot/journal/v4/n7/pdf/nprot.2009.95.pdf
8 s# [- S, d* b" T I% }Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase .7 a9 P9 u: v; q, Z* l! h" o; v
Nature Protocols 4, 1064–1072 (1 June 2009) | doi:10.1038/nprot.2009.95
$ ], @4 d* j; y, k! K5 h# r- p(5)http://www.nature.com/nprot/journal/v3/n9/pdf/nprot.2008.140.pdf5 X5 L& ^* w- g9 H( b
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification .5 T& Z7 F$ a- G) z' a- C
Nature Protocols 3, 1435–1443 (1 August 2008) | doi:10.1038/nprot.2008.140
, H* N7 R) _' H# g. b2 R/ r. \1 C(6)http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.67.pdf
' Q* ~$ h) M3 |, H! nImproved northern blot method for enhanced detection of small RNA # x3 H9 D, ~5 K" ^
Nature Protocols 3, 1077–1084 (1 June 2008) | doi:10.1038/nprot.2008.67
& F/ \/ D( W% a(7)http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.73.pdf3 i+ Z% \: d8 D0 v& m0 L
Analyzing real-time PCR data by the comparative CT method .% O! k. D1 c$ c( s
Nature Protocols 3, 1101–1108 (1 June 2008) | doi:10.1038/nprot.2008.73
2 b1 I# p. E+ x/ H0 j(8)http://www.nature.com/nprot/journal/v3/n4/pdf/nprot.2008.14.pdf
5 d$ j: L H% OMicroRNA expression profiling using microarrays .
8 ]6 a: w6 x2 i3 I9 W$ S. iNature Protocols 3, 563–578 (1 April 2008) | doi:10.1038/nprot.2008.14 |
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