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(1)http://www.nature.com/nprot/journal/v5/n3/pdf/nprot.2009.236.pdf( T# X$ H* ~( l& h
Nature Protocols 5, 516–535 (1 March 2010) | doi:10.1038/nprot.2009.236 4 i" O8 u- j! H& F5 `7 ~
RNA-Seq analysis to capture the transcriptome landscape of a single cell.# |, X1 I2 V- A" T7 a# e* T5 V# n) {
(2)http://www.nature.com/nprot/journal/v4/n11/pdf/nprot.2009.119.pdf
. g5 a9 ^* ^% wA reporter gene imaging system for monitoring microRNA biogenesis .
* ?/ c2 u3 K: I# q; s1 yNature Protocols 4, 1663–1669 (1 October 2009) | doi:10.1038/nprot.2009.119
1 ?+ e; W7 d0 |- H(3)ttp://www.nature.com/nprot/journal/v4/n9test/pdf/nprot.2009.145.pdf6 e+ t$ L! m8 m# E
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression .3 I. W8 t: [1 q: l1 Y
Nature Protocols 4, 1338–1348 (1 August 2009) | doi:10.1038/nprot.2009.145 : G# W `# J, F/ ~ v
(4)http://www.nature.com/nprot/journal/v4/n7/pdf/nprot.2009.95.pdf3 V5 [4 l7 g: W G
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase .6 X$ v0 c3 [( B5 Z
Nature Protocols 4, 1064–1072 (1 June 2009) | doi:10.1038/nprot.2009.95
Y( C' ?" s+ O* i& V P9 b. s(5)http://www.nature.com/nprot/journal/v3/n9/pdf/nprot.2008.140.pdf5 j7 X5 Z% D0 T! s
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification .- W9 c" q! D) F) d* v9 ]$ F" x6 ?
Nature Protocols 3, 1435–1443 (1 August 2008) | doi:10.1038/nprot.2008.140 6 e8 l4 p# d; X0 b) {8 j) e: t
(6)http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.67.pdf
2 @; g, t) \8 p) `" D; e! s0 PImproved northern blot method for enhanced detection of small RNA
+ j) U$ D, k8 tNature Protocols 3, 1077–1084 (1 June 2008) | doi:10.1038/nprot.2008.67
6 }/ x x" V# `6 Z. p7 o) e. y' f(7)http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.73.pdf' Q6 C% N2 M' Y' p. q- g6 j
Analyzing real-time PCR data by the comparative CT method .
6 x( S& Y1 ]( X# J/ \7 I, xNature Protocols 3, 1101–1108 (1 June 2008) | doi:10.1038/nprot.2008.73 : H% c c2 Q4 i! l3 B) f6 V
(8)http://www.nature.com/nprot/journal/v3/n4/pdf/nprot.2008.14.pdf
, Q5 d7 C, R- E( A' cMicroRNA expression profiling using microarrays .3 ` H, t/ |. P3 g6 E% B0 r! o* B
Nature Protocols 3, 563–578 (1 April 2008) | doi:10.1038/nprot.2008.14 |
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