Enrichment for breast cancer cells with stem properties by differential adhesion

饶冠华

Stem Cells Dev. 2010 Mar 11. [Epub ahead of print]  n6 I8 v4 ]9 B$ \8 g2 }9 x
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Enrichment for breast cancer cells with stem/progenitor properties by differential adhesion.
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SIU Med Schoool, Pharmacology, Springfield, Illinois, United States; vwalia@siumed.edu.
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. P  `& n1 e# Y+ ]  S: d; T; vCancer stem cells are commonly isolated by cell sorting for surface antigens that typify stem cells. This technique is very expensive, requiring advanced, high-speed sorters and high-quality antibodies, and yields are often low. Some stem cells can be isolated based on ability to exclude dyes, conferred by expression of membrane transporters, but this property is not universal. Mammary stem cells are known to downregulate cell-cell junctions and exhibit mesenchymal behaviors in vitro. We predicted that such cells should be readily detachable from tissue culture plastic and that this might serve as a basis for their isolation from differentiated cells. We found that immortalized or transformed mammary epithelial cells can indeed be resolved into trypsin-sensitive and trypsin-resistant populations. The former are mesenchymal in morphology and expression profile and are enriched in stem cell properties such as mammosphere-forming ability, drug-resistance, and CD44 stem cell antigen relative to the trypsin-resistant population. The latter, in contrast, are cobblestone in morphology, epithelial in expression profile, and deficient in mammosphere formation. After several rounds of differential trypsinization, the trypsin-sensitive pool had eightyfold higher mammosphere-forming ability than the trypsin-resistant population and twentyfold higher than the starting population. This resolution compares favorably with other enrichment methods. Thus, for relatively differentiated epithelial cell types, differential adhesion may serve as an enrichment strategy to increase the stem cell pool for subsequent manipulations.
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kuangfangnan

好文章，新手学习

hany2007

好文章，我最近发现用化疗药物处理后，有一部分细胞会脱壁，聚集成球形克隆生长，只是不能确定是不是干细胞

avril77777

谢谢分享，学习了

yourwish140

很好，谢谢

hcoohboy

回复 1# 饶冠华 $ s  Y* V  k: p, J; \4 H, K+ d

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! l7 f7 j1 I/ ~1 x    先下载下来，我觉得改用这样的方法可能也是无奈之举啊，因为不同细胞系甚至同一个细胞系不同实验室用同一个marker分选的结果都不一样啊~~~

hcoohboy

回复 9# missyoudad
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8 m+ K# a- p; ?9 _    ----（基于）黏附特性差异的（富集）策略？

wzx6660

很学习

饶冠华

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8 z$ f- a- u& q( U. h" z回复 9# missyoudad 3 C+ z1 @% \7 ]6 [; i

+ k8 k( [& ^1 p( n% q* K# O7 @依据（细胞）贴壁性能差异的策略。 大概意思就是根据细胞贴壁能力的差异来制定的实验方案

missyoudad

不胜感谢。