Enrichment for breast cancer cells with stem properties by differential adhesion

饶冠华

Stem Cells Dev. 2010 Mar 11. [Epub ahead of print]% O$ J# V, R  z8 ^' b* K0 a. B4 N
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Enrichment for breast cancer cells with stem/progenitor properties by differential adhesion.) Z1 h" Y+ Z% k
Walia V, Elble RC.0 i5 m6 k5 a  i* E+ c

9 E2 [% u& u9 i9 m9 u- [SIU Med Schoool, Pharmacology, Springfield, Illinois, United States; vwalia@siumed.edu." M9 u6 \; `, L; a
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Cancer stem cells are commonly isolated by cell sorting for surface antigens that typify stem cells. This technique is very expensive, requiring advanced, high-speed sorters and high-quality antibodies, and yields are often low. Some stem cells can be isolated based on ability to exclude dyes, conferred by expression of membrane transporters, but this property is not universal. Mammary stem cells are known to downregulate cell-cell junctions and exhibit mesenchymal behaviors in vitro. We predicted that such cells should be readily detachable from tissue culture plastic and that this might serve as a basis for their isolation from differentiated cells. We found that immortalized or transformed mammary epithelial cells can indeed be resolved into trypsin-sensitive and trypsin-resistant populations. The former are mesenchymal in morphology and expression profile and are enriched in stem cell properties such as mammosphere-forming ability, drug-resistance, and CD44 stem cell antigen relative to the trypsin-resistant population. The latter, in contrast, are cobblestone in morphology, epithelial in expression profile, and deficient in mammosphere formation. After several rounds of differential trypsinization, the trypsin-sensitive pool had eightyfold higher mammosphere-forming ability than the trypsin-resistant population and twentyfold higher than the starting population. This resolution compares favorably with other enrichment methods. Thus, for relatively differentiated epithelial cell types, differential adhesion may serve as an enrichment strategy to increase the stem cell pool for subsequent manipulations.
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; S" L) o0 l, G) Q/ V' X/ |PMID: 20222827 [PubMed - as supplied by publisher]
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prettybear

5 ]/ o# h# ?+ s8 v3 ]5 _
9 _6 c0 ^. O: V5 z0 ^9 m学习了。这种方法分离肿瘤干细胞又回归到比较初级的方法。最大的好处可能是会最大限度的保留肿瘤干细胞，尽管不纯。
" e( D5 c* |  V& c9 ^8 p2 j   简便实用的好方法。

prettybear

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* H5 s& I* C2 C  j& W: Y一篇和它相关的文献" Y% i4 z9 g6 I$ A) O# m: y
The Epithelial-Mesenchymal Transition Generates Cells with Properties of Stem CellsCell 133, 704–715, May 16, 2008 ª2008 Elsevier Inc.
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The epithelial-mesenchymal transition (EMT) is a key developmental program that is often activated during
* @% k5 v  Y* U0 T) p/ f  ecancer invasion and metastasis. We here report that the induction of an EMT in immortalized human mammary, g5 E9 N, D, P# g7 j* G
epithelial cells (HMLEs) results in the acquisition of mesenchymal traits and in the expression of stemcell
- O  C$ Y+ @, x  hmarkers. Furthermore, we show that those cells have an increased ability to form mammospheres, a property associated with mammary epithelial stem  A: y7 @5 p, ]! }" y
cells. Independent of this, stem cell-like cells isolated from HMLE cultures form mammospheres and express markers similar to those ofHMLEs that have undergone an EMT. Moreover, stem-like cells isolated either from mouse or human mammary glands or mammary carcinomas express EMT markers. Finally,
+ t: A1 N& A0 }9 Q* n" ctransformed human mammary epithelial cells that have undergone an EMT form mammospheres, soft agar colonies, and tumors more efficiently. These findings illustrate a direct link between the EMT and the gain of epithelial stem cell properties.
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饶冠华

回复 3# prettybear
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呵呵  嗯 这篇文章很不错哈  weinberg实验室近几年发的文章我基本上都看过一遍了。 肿瘤细胞发生EMT事件还是他实验室最先发现的哈

prettybear

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( [& B7 l; l$ {( ~- O回复 4# 饶冠华
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4 j( V8 v! V, a6 \' f% @       痛苦的是我最近打算做这方面的文章，却发现别人已经做到很前面了。看来整整的落后了四年。你哪里还有他们实验室这方面的其他文章全文吗？谢谢。

饶冠华

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$ p6 c3 ~5 X: w3 U回复 5# prettybear - z0 I3 _! q' y! R* M( f8 m

6 [' b& U( e7 b: x9 P. b哦 你下不到吗？ 还蛮多很好的文章，记得09年他们每个月一篇NCS的文章

prettybear

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: Y* h) s0 B2 [: }( h- I最近你发的文章都下载了。谢谢你的分享。

missyoudad

thank you

missyoudad

很实用哦。但是能不能告诉我differential adhesion strategies的中文翻译应该是怎样的？

missyoudad

不胜感谢。