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We performed three additional experiments using CD271# h; @; d) X# ?4 d
for enrichment of MSC. Upon isolation of MNC by Ficoll
$ T( |5 Z5 U, h+ Jdensity fractionation, half of the sample was processed/ [# d9 {! e; p- v% F7 K
further using the CD271 MicroBead Kit. About 5% of the cells were in the positive fraction and about 50% of these
2 y0 V' r& M- n3 F8 }1 \! [$ swere CD271
, as determined by flow cytometry. CFU-F4 D& K; [3 `8 I/ X( s2 G
were exclusively found in the CD271
fraction, as
. \% Y% Z: |# i) j+ o0 {described elsewhere [22]. The number of CFU-F per1 B% A6 Z4 B  ]$ a+ \. H% p
cell was 210 times higher in CD271
cells in comparison' B; I# R. t* W) l
with MNC. Hence MSC were enriched in the CD271

, B  S' o0 d/ F$ o) i# }5 bfraction, although the yield of CFU-F per milliliter BM, ^/ i9 S1 [) O! G7 ?. L; V
aspirate or with regard to the number of MNC was greatly# _1 i! p1 }; b5 h
reduced.