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由日本理研的牛人斎藤通紀做的PGC形成信号通路牛文，这个不仅对PGC发生机理研究很重要，而且为ES，iPS分化提供理论基础，也为将来再生医疗提供了重要理论基础。8 \8 `3 f$ x8 f! a
同期Cell专门配发了对本文的评论/ ]7 V8 K% @) L% q m5 w
在此狂赞一把牛人齐藤，愿年纪轻轻的他再创辉煌！
4 G' f! S1 }9 R+ o" k. lhttp://www.cell.com/retrieve/pii/S0092867409002748/ {$ B; k5 d% }" e2 W
Cell. 2009 May 1;137(3):571-84.$ s2 N7 d( F" @- v6 s% ~
A signaling principle for the specification of the germ cell lineage in mice.
3 k4 }2 L- y' H, v" HOhinata Y, Ohta H, Shigeta M, Yamanaka K, Wakayama T, Saitou M.+ G) |- O9 I3 q3 z8 S: K/ e E
Center for Developmental Biology, RIKEN Kobe Institute, Minatojima-Minamimachi, Chuo-ku, Japan.
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Cell. 2009 May 1;137(3):398-400. ]; R4 S- |3 v& _2 o) |* E
http://www.cell.com/retrieve/pii/S0092867409004413
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Specification of the germ cell lineage is vital to development and heredity. In mice, the germ cell fate is induced in pluripotent epiblast cells by signaling molecules, yet the underlying mechanism remains unknown. Here we demonstrate that germ cell fate in the epiblast is a direct consequence of Bmp4 signaling from the extraembryonic ectoderm (ExE), which is antagonized by the anterior visceral endoderm (AVE). Strikingly, Bmp8b from the ExE restricts AVE development, thereby contributing to Bmp4 signaling. Furthermore, Wnt3 in the epiblast ensures its responsiveness to Bmp4. Serum-free, defined cultures revealed that, in response to Bmp4, competent epiblast cells uniformly expressed key transcriptional regulators Blimp1 and Prdm14 and acquired germ-cell properties, including genome-wide epigenetic reprogramming, in an orderly fashion. Notably, the induced cells contributed to both spermatogenesis and fertility of offspring. By identifying a signaling principle in germ cell specification, our study establishes a robust strategy for reconstituting the mammalian germ cell lineage in vitro. |
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发表于 2010-4-9 07:27
