鸟类生殖干细胞的培养

gxmwzy

请问鸟类生殖干细胞研究的意义与前景？
- r  {* l! c! v! h- X# I/ X' g, Y还有，鸟类生殖干细胞的培养和哺乳类的培养是否相似，培养方法是否已经建立？
, t( V5 d; Q6 p  [. ^; [请大家指教

ppcl2

请问鸟类生殖干细胞研究成果可以用于物种保护，也可以生产含某种重要蛋白的转基因蛋啥的，前景也还行吧。/ B  D  ~" G1 h& Y
鸟类生殖干细胞的培养方法在国内外都已建立，和哺乳类的培养有相同也有不同，相同在于，鸟类是建立在哺乳类之后，大量借鉴了哺乳类的培养方法，不同在于发育系统，一个是卵生，一个是 胎生，注定了炒作方法的不同，鸟类取X期胚盘诱导ES细胞，20多期血液中提取PGC来培养EG细胞。# c# M. n0 p* }! ?$ }
具体你可以查文献，中英文都应该有不少。

ppcl2

更正一下，由于很多年没留意这些东西，忘了。
- Q, W6 ~/ X/ @取鸟类PGC应该在Hamburger–Hamilton stages 13–16 (培养大概50个小时左右)的血液和stages 26–32 (培养 4.5到 7.5天)的性腺分离。

gxmwzy

请问一下能不能给我介绍一些鸟类干细胞的文献呀？谢谢

gxmwzy

回复 3# ppcl2
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5 z- s) V- B1 w, L& f请问一下能不能给我介绍一些鸟类干细胞的文献呀？谢谢

ppcl2

回复 5# gxmwzy
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/ X$ G7 z2 e; c0 K- ^6 c  Q, _! I! j8. A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken.
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gxmwzy

谢谢