鸟类生殖干细胞的培养

gxmwzy

请问鸟类生殖干细胞研究的意义与前景？6 R- U9 }; w, C6 k( y" n7 y$ y. ^
还有，鸟类生殖干细胞的培养和哺乳类的培养是否相似，培养方法是否已经建立？
5 r2 ~4 f% c! N$ ?请大家指教

ppcl2

请问鸟类生殖干细胞研究成果可以用于物种保护，也可以生产含某种重要蛋白的转基因蛋啥的，前景也还行吧。
' e9 g9 Q; E# f$ M1 [鸟类生殖干细胞的培养方法在国内外都已建立，和哺乳类的培养有相同也有不同，相同在于，鸟类是建立在哺乳类之后，大量借鉴了哺乳类的培养方法，不同在于发育系统，一个是卵生，一个是 胎生，注定了炒作方法的不同，鸟类取X期胚盘诱导ES细胞，20多期血液中提取PGC来培养EG细胞。
( n5 c! V/ @' o, n* E, ?+ ?9 C7 Q具体你可以查文献，中英文都应该有不少。

ppcl2

更正一下，由于很多年没留意这些东西，忘了。
2 U7 ^: x: h) u. Y3 Y9 z0 h取鸟类PGC应该在Hamburger–Hamilton stages 13–16 (培养大概50个小时左右)的血液和stages 26–32 (培养 4.5到 7.5天)的性腺分离。

gxmwzy

请问一下能不能给我介绍一些鸟类干细胞的文献呀？谢谢

gxmwzy

回复 3# ppcl2
2 b5 _# V4 q  L  [7 \
' T! c6 x9 S% f$ {6 e, l# n请问一下能不能给我介绍一些鸟类干细胞的文献呀？谢谢

ppcl2

回复 5# gxmwzy 8 {, ^! ~3 o5 z5 `6 z' o

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gxmwzy

谢谢