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本帖最后由 细胞海洋 于 2010-4-19 11:09 编辑
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, d8 ? d7 R% F0 ]) ~6 cCell culture is an invaluable tool for investigators in numerous fields. It facilitates3 W; \: Z# p1 D+ e, l: L5 J
analysis of biological properties and processes that are not readily accessible at the level
3 F$ i8 n0 z( y/ `8 [5 Q) _$ wof the intact organism. Successful maintenance of cells in culture, whether primary or
7 |9 u& W5 X1 V' simmortalized, requires knowledge and practice of a few essential techniques. The purpose
# A$ _8 _5 b& T. u# I6 x' ?: qof this chapter is to explain the basic principles of cell culture using the maintenance
6 Q7 T, C7 l, m0 k5 ~of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation) b, M& f8 B @
and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples.
" h# u4 m8 t8 ]7 F4 O/ x1 CProcedures for thawing, culture, determination of cell numbers and viability, and
- ~/ S( b2 [% U0 ^) X! G4 Ycryopreservation are described. |
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发表于 2010-4-19 10:40
