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本帖最后由 细胞海洋 于 2010-4-19 11:09 编辑
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& ~- o' r" d4 O" `& [3 l2 c% ~ Z; LCell culture is an invaluable tool for investigators in numerous fields. It facilitates" G, O1 Q8 S- [' Y* S
analysis of biological properties and processes that are not readily accessible at the level
, K' n B* h/ nof the intact organism. Successful maintenance of cells in culture, whether primary or4 b: _: S0 u. {- u4 |& t. o
immortalized, requires knowledge and practice of a few essential techniques. The purpose
2 z' _5 p' W" m! v x$ gof this chapter is to explain the basic principles of cell culture using the maintenance
7 u) }7 W l9 r0 ]/ {, x3 ^of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation$ k- J( z0 @" [/ Q: m. J2 j. j
and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples.
. h3 f3 n! w% G5 a( ~% yProcedures for thawing, culture, determination of cell numbers and viability, and2 ?/ x& P4 Q# G- c
cryopreservation are described. |
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发表于 2010-4-19 10:40
发表于 2010-5-25 10:08
