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本帖最后由 细胞海洋 于 2010-4-19 11:09 编辑 5 t$ f' R3 W3 `& r9 P
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) ~8 O0 I6 S% `* r; ^Cell culture is an invaluable tool for investigators in numerous fields. It facilitates
1 s6 w; T9 T& P9 Wanalysis of biological properties and processes that are not readily accessible at the level
# O- b9 v; d* U5 ?) ] }; h8 }% Xof the intact organism. Successful maintenance of cells in culture, whether primary or
( t3 w* m. r% V8 M, L' pimmortalized, requires knowledge and practice of a few essential techniques. The purpose
/ m( G. b0 X! R8 N* q- A% j5 R5 Aof this chapter is to explain the basic principles of cell culture using the maintenance
% S2 f$ t7 r) z; V8 o( X" }' Q3 |1 Qof a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation
8 ]) t, _/ B* d ]! ~1 W+ |and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples., s9 e; S, `/ w- G) \# ^
Procedures for thawing, culture, determination of cell numbers and viability, and
; o! j; C2 y9 j, v+ N* G8 c& |+ Dcryopreservation are described. |
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发表于 2010-4-19 10:40
