|
- 积分
- 50
- 威望
- 50
- 包包
- 301
|
本帖最后由 细胞海洋 于 2010-4-19 11:09 编辑
. |- S4 ^; E; p2 ?" G
0 a, D4 W, M; W1 `, A[hide][/hide]
! \2 ]& F* y7 S, c2 p5 a
2 C( F1 h) C0 p/ W5 i; r {Cell culture is an invaluable tool for investigators in numerous fields. It facilitates
7 I) r8 w& J3 n* |analysis of biological properties and processes that are not readily accessible at the level
. W( F [9 g9 g1 z1 oof the intact organism. Successful maintenance of cells in culture, whether primary or
* e) j/ m: y* S. mimmortalized, requires knowledge and practice of a few essential techniques. The purpose
5 n2 j# M7 G$ A4 z* Pof this chapter is to explain the basic principles of cell culture using the maintenance
( S; O5 r/ a4 C- x0 p @6 yof a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation
3 h U! j0 J0 V8 |" x9 G1 D$ R: _" Fand culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples.
- P" H. ]; ?+ T9 }Procedures for thawing, culture, determination of cell numbers and viability, and
- L/ P# J/ [1 x- I* h" K' W6 F% jcryopreservation are described. |
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
-
总评分: 威望 + 5
包包 + 10
查看全部评分
|
发表于 2010-4-19 10:40
发表于 2010-5-25 10:08
