干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

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干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf
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【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—
7 g# ~0 m5 r! g* cSCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植  @; _1 p) Q. e# m6 ^- u" {0 W
组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存
( \! G5 V" l7 H+ j活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡
- z1 K; n: o, w; k! F) m0 a2 Q细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，) g( M# `, O; [: _4 z
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗
7 t& B0 }) n1 _# n死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上9 q4 }: a: d$ w, B
升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌' }) @- A+ S" t% |
样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。
, {" h& D1 D' t* \6 u【关键词】干细胞移植心肌梗死心力衰竭
# U$ g: S5 x6 T4 b/ i0 Z. [The Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU4 {% k, O- z8 U) y1 J
iyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—- n$ k+ ?1 ]8 o! w
niversity College of Medicine，Hcmgzhou 310009,China
+ b4 F' p" z, x* S7 s, \( N【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—$ ^1 @* Q+ y" A
tion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—0 u6 R5 R( ~6 a8 {( a  n9 F' u
nines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm
* z# q; }9 ]9 M5 o2 u: x( fand ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group
3 L4 w( q/ E' j1 Rat 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—  ^6 C3 M4 f( E5 ~
entiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and
) A& Z; T& x6 Napoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．4 H6 Y/ i6 X" K& h
Smwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——
5 ]7 C. @4 x1 q9 S. \oeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal
/ \/ c% O0 e) p, d+ Sindex、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—2 E# o( z% b1 }" v. E
trol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．, I; `9 z! G2 B! n8 X
Conclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible% @$ @+ L3 j- @
reason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—- @8 P( z! G2 d( H* J8 G
tion collogen deposition in noninfraetion zone．
) n: ?; x4 p( z& O$ F  F: F【Key words] Stem cells Myocardial infarction Heart failure
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