干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

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干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf
# U9 U% V9 d4 u2 Q; d徐赤裔周建庆方建江廉孝芳( x7 r8 ^6 j0 |" T. b/ o( U
【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—
$ s! g- E, p% ^8 `& X+ MSCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植
# U5 I/ g) ]2 U' f组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存
4 H" {% @" Q! _9 P& I7 b6 F活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡
% Z9 a; c+ ^" ?: I细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，7 v, m* i# Y  [# v( |8 @; I. L
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗: n1 I- I3 g' ?: C" J0 q
死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上/ [+ c0 L; O- M5 \% M+ n" M# z
升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌( Q) C$ W$ h4 D! x; U
样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。
' k8 B' Z3 E, A" E1 e【关键词】干细胞移植心肌梗死心力衰竭; y, y* c3 A( L
The Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU
1 M; o; @8 r! P5 a9 \  H( Z( \iyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—
& X/ a% g" g. J' Sniversity College of Medicine，Hcmgzhou 310009,China
3 k2 ]0 G1 _, r【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—
! L' p1 z0 d. z9 m: Qtion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—% h; s/ i% G1 \- n+ G& j0 r- n
nines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm
& A6 a; r0 B# x2 D+ j6 M" |and ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group) R3 \6 m& w# F) W0 z0 M0 d
at 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—% [2 ~  M2 M6 Z& C& g
entiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and
1 M0 k' U, C% u' A: Z: kapoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．5 s8 E0 X* u( ^- I8 z6 z7 w
Smwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——
) |, r3 ]3 J! R0 S9 J9 G% g+ y  Koeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal- c7 h8 S7 w& O. `: X/ p& ^
index、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—" z: m" `4 y2 t3 p9 l
trol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．3 l5 A% T. J* j9 \
Conclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible
$ n9 d! r0 ^4 }7 ~8 X! ]6 Freason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—" L4 }6 D* Z; T) Y
tion collogen deposition in noninfraetion zone．
+ ?# T, V% M, K: H) d# y- x【Key words] Stem cells Myocardial infarction Heart failure
  X/ T/ s' T; K0 U6 n! r[hide][/hide]

月飘东海

我要看看嘿嘿

siwa

很好，参考一下，谢谢

ligangcangxb

可以参考

在路上

1# 海人 [/b谢谢

luohaijian

哥吃的不是面

天空为我蓝

回复 1# 海人
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5 D0 b) a! y$ H$ F+ d# }    谢谢,看看

桂圆儿

谢谢

study

想看看，谢啦