干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

海人

干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf. Q; J# E' E) G- @: u, [) k
徐赤裔周建庆方建江廉孝芳
' T& ^1 I: ]( d  Y5 @【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—' s( `0 i2 H. }% h& u9 s0 \
SCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植
& M* j! C# l7 G' W  [+ x6 q( E8 t组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存
/ {" z) e0 a; H/ Y- y活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡
) F5 m7 W2 b5 n* ~0 [. \) m细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，: e3 ]" k7 W% H* ^: R
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗
( c$ E- b5 R+ u/ N! H5 e' m死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上
# c  `; h6 B4 |  R9 J4 d升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌) Y7 o) p+ x) h7 e' }8 G! |
样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。5 }) u3 ~( ^. |/ x* z
【关键词】干细胞移植心肌梗死心力衰竭
) D* |8 w. S4 n& R, tThe Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU) P- P1 b  X$ }9 j6 Y/ b5 G
iyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—5 |/ z; [# U' Z( O) W4 N
niversity College of Medicine，Hcmgzhou 310009,China2 J* W0 s7 o) {: n
【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—
  A3 \3 _; G, u( u# s3 m# x4 J- \3 Vtion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—) |5 z6 p8 J' Y& M! K
nines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm
  l2 m- `4 Z$ g2 @and ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group
! Q4 T3 h, U3 Z% N4 d8 W9 pat 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—
! b5 R5 W# m! L# }8 i4 x& rentiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and5 m0 j$ S5 g7 t" {. L1 ]% y
apoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．
6 v% O) a, a3 l2 s5 mSmwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——
: r+ P- B* G: x, Q" G" G- R% coeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal1 ~' z9 e; N2 K6 c
index、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—& p# [$ R1 s& N7 g0 K
trol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．( r& B- C- i5 k" P7 ]
Conclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible
; H# @  Q/ z5 X# T$ q7 Ireason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—3 R/ K9 @: y. u1 w5 m* s8 R0 N1 z# p$ m
tion collogen deposition in noninfraetion zone．! Q* l* ?' F) D) D$ u
【Key words] Stem cells Myocardial infarction Heart failure
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月飘东海

我要看看嘿嘿

siwa

很好，参考一下，谢谢

ligangcangxb

可以参考

在路上

1# 海人 [/b谢谢

luohaijian

哥吃的不是面

天空为我蓝

回复 1# 海人
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- V6 r# z$ F5 H6 M    谢谢,看看

桂圆儿

谢谢

study

想看看，谢啦