干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

海人

干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf
# r- v; ?/ H5 M/ B+ x' S9 v徐赤裔周建庆方建江廉孝芳) q! M# r7 @; F5 b5 l7 T2 i$ v4 a
【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—
- G) Q. O5 @- ?% ?& h  \SCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植
* ?! U1 G* `( ?4 F组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存
+ U/ X4 U- v- U+ t活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡
0 v9 z' {, w' M  Q, c9 C, D细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，; C  s( d' z" B4 S4 j1 b5 k9 @
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗
! f) I: H/ ?& d死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上
; U$ I5 V0 j2 _* ^升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌+ j- J) Z- `) O) R! }! L6 w2 [& o: h# h
样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。# `0 M) F4 ^" h, A. M5 _
【关键词】干细胞移植心肌梗死心力衰竭
- O, T- g# q2 T1 s9 Y$ lThe Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU
  f0 @0 T' \# m; c, ziyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—3 o/ R& X! `7 |- R" D# t1 I% U
niversity College of Medicine，Hcmgzhou 310009,China2 g& r1 [/ O, A5 R
【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—
7 D9 x/ J" O! U! Q& Dtion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—5 b8 Q! @+ L( b, Q; [4 ?8 O& \0 f* ]1 ?
nines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm( a. l0 S! p* i- L  r, g
and ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group5 t: c9 p: j0 W4 C, Q+ ^# d) Q4 ?$ x
at 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—/ D7 f5 I- y: D( l5 c
entiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and
! o8 v) I- `. Q% H. aapoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．
9 e: D( F% e/ {+ C( N  LSmwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——3 ]- {+ V% O& e% u4 L" x3 o
oeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal
* [/ l# i* h. P" L/ j+ P4 Findex、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—8 b9 b" s8 v5 l3 _+ f/ _5 a
trol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．+ b: X& _( P6 u( o! T1 N
Conclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible4 E" S) t0 @# b; W  G/ _- @0 L
reason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—
# |+ G& `9 f1 y7 `6 X8 ltion collogen deposition in noninfraetion zone．
( h  d. f( E) v7 p【Key words] Stem cells Myocardial infarction Heart failure  k* ]3 v/ ?" h9 Y* }
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想看看，谢啦

桂圆儿

谢谢

天空为我蓝

回复 1# 海人 . z" {  B, y" C; Q
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    谢谢,看看

luohaijian

哥吃的不是面

在路上

1# 海人 [/b谢谢

ligangcangxb

可以参考

siwa

很好，参考一下，谢谢

月飘东海

我要看看嘿嘿