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knockout DMEM是什么意思,knockout血清替代品呢     [复制链接]

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楼主
发表于 2010-5-14 16:24 |显示全部帖子
knockout是一个品牌,knockout DMEM是一个基本培养基
5 [6 J" w: K5 d2 s0 h& jksr是一种成分明确的无血清配方,可直接替代现行方案中的胎牛血清
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具体如下/ I  u  g7 W% ^; ?; b: Y
1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.3 Z9 `/ r0 H# ?
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture
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沙发
发表于 2010-5-14 16:59 |显示全部帖子
不谢
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