knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基3 R/ v+ v+ e$ {0 p. ^$ d% D. J
ksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清* P9 \, a0 v( E" ]' |3 m. U- j
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具体如下
+ t  B1 f2 n2 g8 Z9 f. M1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.1 |2 O0 y3 g9 J6 d9 \
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe ; _" X  h9 C4 u. I: c
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$ ?! F. J  A% f- }0 B    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基
& `$ c, U; ~- H2 c5 U% ]ksr是一种成分明确的无血清配方，可直接替代现行方案 ...
8 ^( K. w7 z* e3 i2 fdaviehe 发表于 2010-5-14 16:24

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3 F2 ?: p: G0 K3 e   谢谢解答！

znhy

回复 5# lifescience1 ! T+ G* Q" V9 D. _5 ?8 v
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很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確; {% l# p4 q( G
但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份
7 w/ Y% n* V+ j1 R2 K4 N只提到專利申請中(可能怕使用者自己調配吧）
% ]* ^& z" D+ K* x4 @+ n也就是說- t, V5 W# ^) Z6 m- C8 c! g
成份明確，但是不讓你知道....

简简单单

好，学习了