knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
* n8 F& @; }5 mksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清! p% P# a" k4 z( v) N
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具体如下
7 J' s* |/ P2 U7 @3 s1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.) {% Q0 K5 K8 e4 g$ r
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe ( A* ]* J6 v. z( N# U& Q7 Z3 L
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    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基
, d) v2 c) @# ]7 n6 nksr是一种成分明确的无血清配方，可直接替代现行方案 ...
7 _- z/ A2 p9 _' Y/ P( t7 ~daviehe 发表于 2010-5-14 16:24

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   谢谢解答！

znhy

回复 5# lifescience1
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  F9 d  C0 r9 y& F$ R% ]9 ~4 b很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確
6 |7 w+ h& ]  n. y, z! q但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份8 P6 z1 N- T! M5 k/ v
只提到專利申請中(可能怕使用者自己調配吧）
7 `5 P- H6 p1 D7 c- b! O也就是說
# o6 Z' q4 r5 P: f; _8 i* _# k成份明確，但是不讓你知道....

简简单单

好，学习了