Embryonic Stem Cell Lines Derived from Human Blastocysts

ng_pan

+ T* c: n% `- o
4 w; H8 `5 f" ?& A  P/ L
Embryonic Stem Cell Lines; D( M/ w( M% x& Y
Derived from Human
) ~1 K* q' O/ K( a" r1 jBlastocysts
. B0 k' z3 K2 C8 Q3 DJames A. Thomson,* Joseph Itskovitz-Eldor, Sander S. Shapiro,
4 p8 s! b$ p) W9 c7 k$ u6 B3 M7 wMichelle A. Waknitz, Jennifer J. Swiergiel, Vivienne S. Marshall,
: b1 S" X0 o; M5 O7 ?5 v, G- [8 aJeffrey M. Jones9 G( m. Q' Y* P
Human blastocyst-derived, pluripotent cell lines are described that have normal! \2 n" s' t1 g$ P4 o: ^
karyotypes, express high levels of telomerase activity, and express cell surface( i, I8 ]5 ?4 v0 v# |: \4 ^
markers that characterize primate embryonic stem cells but do not characterize9 T! m- F' ]- l3 ~
other early lineages. After undifferentiated proliferation in vitro for 4 to 5
/ c% e' c/ c3 N. a/ O0 zmonths, these cells still maintained the developmental potential to form trophoblast
, B: O0 H0 j' S  I8 Eand derivatives of all three embryonic germ layers, including gut
! f" g1 c6 K/ C5 d' A7 ^epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle
( ~  v3 O: N$ |2 \(mesoderm); and neural epithelium, embryonic ganglia, and stratiÞed squamous
* Y9 S/ m5 P1 Z" T1 Tepithelium (ectoderm). These cell lines should be useful in human developmental
5 \: ~, Z; Q! T$ t! abiology, drug discovery, and transplantation medicine.
. R% C/ G, A& w4 Y! a2 }" d0 Z6 f! d. y. R4 q: j; E
附件为原文 请回复后下载
8 i. {8 }2 u) |% `9 M0 ^
8 j: b0 I! b6 a9 `) F; h0 q! a2 y