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本帖最后由 细胞海洋 于 2011-10-20 15:38 编辑 2 V I- l8 t: A: l6 |3 u
; S# @! ?9 j7 h3 ?! z9 @ g王公平1,2,杨波1,关方霞3,杜英4,常克亮1,宋来君1,胡祥5,曾光伟1,20 H/ G0 r+ `6 R7 r
(1.郑州大学第一附属医院神经外科,郑州450052;2.河南科技大学第一附属医院肿瘤科,' Y+ x5 a6 P2 s/ W6 i7 L) F
河南洛阳471003;3.郑州大学生物工程系,郑州450001;4.郑州大学基础医学院微生物0 _1 r8 Y8 S: ], Q' M# R4 _
与免疫学教研室,郑州450052;5.深圳市北科细胞工程研究所,深圳518000)9 Y; u" I) T' |, u) q
[摘要] 目的:通过不同途径移植羊膜间充质干细胞治疗脑损伤大鼠,比较脑损伤大鼠行为学的改! M1 z! ?+ _" z' L; a% e$ c4 A" t
善,为脑损伤治疗选择有效的移植途径。方法:制作80只Wistar大鼠脑损伤模型,分为对照组与治疗
# h/ _1 D' K0 u/ D4 y" ^4 i组。对照组为单纯脑损伤大鼠,共20只;治疗组分为尾静脉移植组(A组)、侧脑室移植组(B组)、脑损# v. u2 ^, i) J7 W
伤区移植组(C组),每组20只,均给与植入人羊膜间充质干细胞,采用NSS评分法评定治疗效果。结
* @% {) I- |: _( k# q3 v果:脑损伤区移植组大鼠行为学改善与其他各组相比,差异有统计学意义(P<0.05);侧脑室移植组分
% i% z+ z# W& [+ Q5 k8 Y别与尾静脉移植组及对照组相比,差异有统计学意义(P<0.05);而尾静脉移植组与对照组相比,差异' I6 M' @. {0 M
无统计学意义(P>0.05)。结论:脑损伤区移植羊膜间充质干细胞是治疗脑损伤大鼠的有效途径。
- J& R3 c+ D) @0 u3 r$ G[关键词] 羊膜间充质干细胞; 移植; 大鼠; 脑损伤
9 h$ j1 k: H6 v; u* m[中图分类号] R651.65 [文献标识码] A [文章编号] 16727347(2008)10092605
2 M- U9 N2 g$ U7 w5 |, a3 d8 u. KTreatmentofbraininjuredratsthroughtransplanting
. X" n) {! A4 q/ bamnioticderivedmesenchymalstem cellsindifferentways8 ?" X1 r( X# X- p! U" m% d# m
WANGGongping1,2,YANGBo1,GUANFangxia3,DUYing4,CHANGKeliang1,$ |. r/ x( ]: h; L% [, y
SONGLaijun1,HUXiang5,ZENGGuangwei1,2
' G9 o8 j9 H7 I0 E+ O1 t4 p$ _(1.DepartmentofNeurosurgery,FirstAffiliatedHospital,ZhengzhouUniversity,Zhengzhou450052;
9 L, o6 c+ e; M+ a/ p2.DepartmentofTumor,FirstAffiliatedHospital,HenanUniversityofScienceandTechnology,
/ r! {9 G% a+ L( e7 mLuoyangHenan471003;3.DepartmentofBiomedicineandBioengineering,ZhengzhouUniversity,+ Y" F# P4 ^. b" _
Zhengzhou450001;4.DepartmentofMicrobiologyandImmunology,CollegeofBasicMedicalSciences,0 M% w: E& a( ?' ~% q8 o
ZhengzhouUniversity,Zhengzhou450052;5.ShenzhenBeikeCellEngineeringInstitute,Shenzhen518000,China)
: d) S( R7 {1 f' T+ j0 aAbstract: Objective Tocomparethebehavioralimprovementtofindthebesttransplantation
" g7 B# y" k7 N( eapproachfortreatingbraininjurythroughtransplantingamnioticderivedmesenchymalstem cellsinto- x {0 i8 I O, V& W+ h
braininjuredratsindifferentways.Methods EightybraininjuredWistaratswererandomlydivided3 ?" H+ l( \( ~/ m8 |
intoacontrolgroupwithbraininjuryalone(n=20)andatreatmentgroup(n=60)whichwere; y* {, F) G1 }) ~4 \
furtherevenlydividedintoGroupA(transplantedthroughthevenacaudalis),GroupB(transplanted
0 `- J5 y O! }7 _3 Sthroughtheventriculuscerebrilateralis), andGroupC (transplantedthroughtheinjuredbrain0 o4 z# }) v1 O6 j) T7 O
area).Eachgroupwastransplantedwithamnioticderivedesenchymalstemcells,andtheirtherapeu
* ^: v% A0 x0 Oticefficacywouldbeevaluatedthroughtheneurologicalseverityscore(NSS).Results Compared withothergroups,thebehaviorsofGroupChadmarkedlyimproved.Therewasstatisticallysignifi. {( v% p0 l4 {" Z
cantdifferenceinthe2groups(P<0.05).Comparedwiththecontrolgroup,thebehaviorsof
7 l2 y- U( }: y' X. E' F: QGroupAandGroupBhadmarkedimprovement.Therewasstatisticallysignificantdifferenceinthe3
4 }5 u& V4 V, p3 o' n* t9 pgroups(P<0.05).However,therewasnosignificantdifferencebetweenGroupAandthecontrol) ~6 t3 L* w) [) _
group(P>0.05).Conclusion Transplantingtheamnioticderivedmesenchymalstemcellsintothe
, h$ r9 ~. d- W% e" n" tinjuredbrainareamaybeeffectiveforbraininjuryinrats.
" S g, t0 D) t% | [6 CKeywords: amnioticderivedmesenchymal; stem cell; transplantation; rats; brain$ A' P: b' e) e: v
injury# H' s$ t8 x& F4 t
[JCentSouthUniv(MedSci),2008,33(10):092605]" Z; b$ y& [# Z# x3 L
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