成鼠骨髓间充质干细胞分化为神经元样细胞的研究

kuaileren

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3 c# m- @" |  p+ F. x8 ?, C9 y7 n袁维1 ,路磊1 ,刘宏志2 ,芮刚1 ,孔令强1
6 x9 r- d) D9 H( |  N(1. 中国医科大学附属第一医院骨科,辽宁沈阳110001 ; 2. 铁法市铁煤集团总医院骨科)
0 d( y$ e* l' q6 _  O& G8 d) {　　【摘要】目的:探讨成年大鼠骨髓间充质干细胞(MSCs) 的体外培养和向神经元样细胞分化的能力。方法: 利
& f3 c# C+ |& ]! {& ^+ H2 v用Percoll 分离液(1. 073 ×103 g/ L) 梯度分离成年大鼠MSCs ,采用3 种不同的方法对其诱导分化,免疫细胞化学方
! B! Q! W" V$ [7 b; m法检测神经元特异性烯醇化酶(NSE) 、神经丝蛋白(NF) 、星形胶质细胞特异性标志( GFAP) 和神经前体细胞
* ?  L  I, i0 [  N1 }0 o. ?(Nestin) 的表达。结果: 成功进行了成年大鼠MSCs 的原代和传代培养,3 种诱导方法均能使MSCs 分化为神经元  N' X. m4 ?; N: i
样细胞,都能表达NSE、NF 和Nestin ,而不表达GFAP。结论: MSCs 能在体外分离、扩增、传代,并能向非间充类细
+ T( c' X6 X0 [6 r* B胞分化。: D4 c: v0 y+ Z, s, ^8 q
【关键词】骨髓;细胞培养;间充质干细胞;诱导分化;神经元样细胞7 y! t2 k  T! s, ?" |4 W5 [
【中图分类号】R681 ;R338 　　　【文献标识码】A 　　　【文章编号】0258 - 4646 (2004) 02 - 0121 - 024 S7 _7 d  u8 F; J2 E; y1 [
In vitro culture and differentiation into neuron2l ike cells of
$ N1 J6 B. t4 I0 v# Omesenchymal stem cells in adult rats
3 p( p7 l% m4 [- x. G/ T4 q5 aYUAN Wei1 , LU Lei1 , L IU Hong2zhi2 , RUI Gang1 , KON G Ling2qiang1& _/ }% N# H% |8 g: z
(1. Department of Orthopaedics , The First Affiliated Hospital , China Medical University , Shenyang 110001 , China ;
: O0 D6 ~  ^( q0 j- k; C$ v5 x3 s2. Department of Orthopaedics , Tiefa Coal Group Hospital)
% [/ f+ {1 x9 K3 A5 A7 a0 S; m7 K【Abstract】　Objective : To study the isolation , purification , and differentiation of mesenchymal stem cells (MSCs)
1 H# s; H' G0 y" [- w( m6 t: ^into nerve cells in rats in vitro. Methods : Bone marrow mononuclear cells from femur and tibia of rats were obtained ster24 O- Q- P- Y/ J1 E" w  |; f+ [! e
ilely with gradient centrifugation by using Percoll (1. 073 ×103 g/ L ) . The low density cells including MSCs were culti2
: F0 `, w3 i) u  E7 {0 _vated and induced to differentiate into nerve cells with 3 different treatment protocols. Neuron2specific enolase (NSE) ,0 [; ~0 W* N: j# i: L: {
neurofilament (NF) , glial fibrillary acidic protein ( GFAP) , and Nestin were detected by using immunocytochemistry" x3 {) Z+ F* `/ N) M
method , and quantitation analysis of neuronal differentiation. Results : The MSCs were successfully cultivated primarily4 b$ h8 d7 ~( p
and secondly. All 3 treatment protocols induced MSCs to differentiate into neuron2like cells. The neuron2like cells ex2, h- B# Y. r; p% {( J6 C
pressed NSE , NF , and Nestin ,but could not express GFAP. Conclusion : The MSCs can be cultured and passaged in vitro0 g3 ]/ I5 F4 _
and induced to differentiate into non2mesenchymal cells.4 f) p* d3 u8 D  D1 f. D
【Key words】　bone marrow ; cell culture ; induction differentiation ; neuron2like cells ; mesenchymal stem cells, X5 [6 ?2 ~$ e) B" H
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张俊克

好文章，谢谢

张俊克

好文章，谢谢]

shishi

thanks

张俊克

好文章

22002393

3Q3q

zhengyaguo

谢谢分享

zhengyaguo

谢谢分享

sdyinong

1# kuaileren 2 e* F2 k0 P. M8 J" h* `
- J' i# Z7 P: \1 b. Q% \/ [
谢谢

圆圆yy

谢谢