成鼠骨髓间充质干细胞分化为神经元样细胞的研究

kuaileren

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2 [7 J$ f! X4 k* r7 M3 f5 H$ v" C袁维1 ,路磊1 ,刘宏志2 ,芮刚1 ,孔令强1
- r5 D; l9 E# H- @  v- B- d(1. 中国医科大学附属第一医院骨科,辽宁沈阳110001 ; 2. 铁法市铁煤集团总医院骨科): M* u4 t) B; A; F0 L
　　【摘要】目的:探讨成年大鼠骨髓间充质干细胞(MSCs) 的体外培养和向神经元样细胞分化的能力。方法: 利4 y: ?9 P8 M% Y& y
用Percoll 分离液(1. 073 ×103 g/ L) 梯度分离成年大鼠MSCs ,采用3 种不同的方法对其诱导分化,免疫细胞化学方
$ I/ F, k) q4 `8 `9 C7 j& ?法检测神经元特异性烯醇化酶(NSE) 、神经丝蛋白(NF) 、星形胶质细胞特异性标志( GFAP) 和神经前体细胞
' `. h, b1 i. M, u(Nestin) 的表达。结果: 成功进行了成年大鼠MSCs 的原代和传代培养,3 种诱导方法均能使MSCs 分化为神经元
  w" Q/ H) P9 b  B( X样细胞,都能表达NSE、NF 和Nestin ,而不表达GFAP。结论: MSCs 能在体外分离、扩增、传代,并能向非间充类细2 q2 C4 ?3 U$ G' m3 ^: K. k
胞分化。  I2 }" R" i( k3 B
【关键词】骨髓;细胞培养;间充质干细胞;诱导分化;神经元样细胞0 Q* C3 z, o' i* i7 u9 ~
【中图分类号】R681 ;R338 　　　【文献标识码】A 　　　【文章编号】0258 - 4646 (2004) 02 - 0121 - 02, y7 G) [7 V9 P
In vitro culture and differentiation into neuron2l ike cells of
% ~. o1 _* l, vmesenchymal stem cells in adult rats
+ d: Y4 I- T$ V1 jYUAN Wei1 , LU Lei1 , L IU Hong2zhi2 , RUI Gang1 , KON G Ling2qiang1/ ?) W% Z+ c. P+ F7 p
(1. Department of Orthopaedics , The First Affiliated Hospital , China Medical University , Shenyang 110001 , China ;8 ~9 ?% `- G/ U' h' V- I+ N
2. Department of Orthopaedics , Tiefa Coal Group Hospital)
- D7 }  S! m1 d# g9 D【Abstract】　Objective : To study the isolation , purification , and differentiation of mesenchymal stem cells (MSCs)
0 [) i1 e+ X& Q) s7 G5 E: r% ?# Ginto nerve cells in rats in vitro. Methods : Bone marrow mononuclear cells from femur and tibia of rats were obtained ster2
4 f6 ]! b3 x3 z; ]1 }/ B% q1 ~ilely with gradient centrifugation by using Percoll (1. 073 ×103 g/ L ) . The low density cells including MSCs were culti2
: w  N$ u) c7 y, v( i$ Ovated and induced to differentiate into nerve cells with 3 different treatment protocols. Neuron2specific enolase (NSE) ,
& {8 b: A4 \; fneurofilament (NF) , glial fibrillary acidic protein ( GFAP) , and Nestin were detected by using immunocytochemistry
1 j, ^6 _8 t- K: q1 ]- y0 J( ?4 a: }method , and quantitation analysis of neuronal differentiation. Results : The MSCs were successfully cultivated primarily& ?3 e1 ~8 X4 l- r! W
and secondly. All 3 treatment protocols induced MSCs to differentiate into neuron2like cells. The neuron2like cells ex23 e6 H) ]6 ?3 _" g3 @" F0 i: b
pressed NSE , NF , and Nestin ,but could not express GFAP. Conclusion : The MSCs can be cultured and passaged in vitro
9 z3 m" |6 [0 K: pand induced to differentiate into non2mesenchymal cells.
& O4 Q+ g$ g. m/ |' K0 a【Key words】　bone marrow ; cell culture ; induction differentiation ; neuron2like cells ; mesenchymal stem cells
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张俊克

好文章，谢谢

张俊克

好文章，谢谢]

shishi

thanks

张俊克

好文章

22002393

3Q3q

zhengyaguo

谢谢分享

zhengyaguo

谢谢分享

sdyinong

1# kuaileren . S# H- m  Q) h# @) Z4 C
; A8 R! D9 _/ U: F+ S( u9 B0 m0 O& o
谢谢

圆圆yy

谢谢