人骨髓间充质干细胞在成年大鼠脑内的迁移及分化

cdk1

) j' E2 p4 s  p! e& w
4 t. A' |9 M- V1 a, j9 K" ?7 L$ e
侯玲玲1 , 郑　敏1 , 王冬梅1 , 袁红丰1 , 李海民1 , 陈　琳1 , 白慈贤1 , 张　涌2 ,4 |9 j- X  P8 n8 b9 a, b1 g
裴雪涛1 , 3
3 ]7 i  J' [6 B4 j9 @9 `- C1 军事医学科学院输血研究所、军事医学科学院干细胞中心, 北京100850 ; 2西北农林科技大学胚胎工程实验室,
$ R' p' C& g- [, V4 g9 e+ V陕西省杨凌712100% e5 d* V  r. H, j
摘　要: 　骨髓间充质干细胞(mesenchymal stem cells , MSCs) 是目前备受关注的一类具有多向分化潜能的组织干细胞,5 v+ E+ F) {/ Q
体外可以分化为骨、软骨、脂肪等多种细胞。因此, MSCs 是细胞治疗和基因治疗的种子细胞之一。为了探索MSCs 的
. j. \7 I& [2 N# W迁移和分化趋势, 为帕金森病(Parkinson disease , PD) 的干细胞治疗提供理论和实验依据, 本实验将体外扩增并转染增# t8 J, A' b, }6 I
强型绿色荧光蛋白(enhanced green fluorescent protein , EGFP) 的人骨髓MSCs 注入PD 大鼠脑内纹状体, 观察了人骨髓3 _) f- F6 h0 r+ l/ r1 q' ~
MSCs 在大鼠脑内的存活、迁移、分化以及注射MSCs 前后大鼠的行为变化。结果表明, 人骨髓MSCs 在大鼠脑内可存
3 h( C/ a! |- ?活较长时间(10 周以上) ; 随着时间的延长, MSCs 迁移范围扩大, 分布于纹状体、胼胝体、皮质以及脑内血管壁; 免疫
8 V* P+ v9 f( g& r组化法检测证实MSCs 在大鼠脑内表达人神经丝蛋白(neurofilament , NF) 、神经元特异性烯醇化酶(neuron2specific eno2
- ^+ f- X% [0 L2 b7 Ulase , NSE) 以及胶质原纤维酸性蛋白(glial fibrillary acid protein , GFAP) ; PD 大鼠的异常行为有所缓解, 转圈数由8186 ±+ f" X/ e) A) M. e
2109 r/ min 下降到4187 ±2106 r/ min , 统计学分析P < 0105 为差异显著。以上观察结果表明, 骨髓MSCs 有望成为治疗( y. k$ ^7 V6 Q. ]/ n% g$ ~
PD 的种子细胞。
; D3 r8 n. v9 N' I% p, q1 w% W关键词: 骨髓间充质干细胞; 大鼠; 脑; 分化; 迁移! V8 B" H! X) ~6 L' T
中图分类号: Q254
: u. R- Z+ O6 c- q; B# K, Q, cMigration and differentiation of human bone marrow mesenchymal stem. X0 S  G7 s$ y; G) Z
cells in the rat brain/ W6 _5 i- H4 R! e2 a
HOU Ling2Ling1 , ZHENGMin1 , WANG Dong2Mei1 , YUAN Hong2Feng1 , LI Hai2Min1 , CHEN Lin1 ,4 J) J( v. n. M8 S! b
BAI Ci2Xian1 , ZHAN G Yong2 , PEI Xue2Tao1 , 3
+ F1 ]* k5 @" U) f3 Y/ J1Beijing Instit ute of Transf usion Medicine , Beijing 100850 ; 2 Northwest Sci2Tech University of A gri20 M6 B6 C+ p0 {$ c8 s7 n( E" m+ `
cul t ure and Forest ry , Yangling , S hanxi 712100
+ i+ N/ D" a3 zAbstract : 　Bone marrow mesenchymal stem cells (MSCs) are multipotent tissue stem cells that can be induced7 W7 @) p; A- l& N
in vit ro to differentiate into a variety of cells such as osteoblasts , chondrocytes and adipocytes. MSCs are useful
& y$ I" X+ f" `. y) n$ |
, W4 a: {& c& v( D- [- f9 g
, A7 |3 s6 X! x5 Y" A+ o& Nvehicles for both cell and gene therapy for a variety of diseases. Here , we injected human MSCs with enhanced2 E  q5 P1 S1 ]) b" z" W
green fluorescent protein (EGFP) into the striatum of Parkinson disease (PD) rat and examined their survival ,1 U/ ~$ L; S# f* m: Z7 O
migration , differentiation , and the behavior changes in PD rats , which will provide a theoretical foundation and
- d: \8 e, |  |" }6 Ftechnical method for clinic PD therapy by stem cells. The results showed that human bone marrow MSCs can
) h. U: `4 n+ S. Jsurvive in rat brain for a long time (exceeding 70 d) . MSCs were found in multiple areas of the rat brain includ2
- }8 _7 q8 ^7 U0 V) ~ing the striatum , the corpus callosum , contralateral cortex and even the brain vascular wall. Immunocytochemical! `5 E& |/ |. s* S
staining suggested that implanted cells expressed human neurofilament (NF) , neuron2specific enolase (NSE) and$ k( y0 R6 o# V6 u" ]4 G
glial fibrillary acid protein (GFAP) . At the same time , remission in abnormal behavior of the PD rats appeared.& L- @: ~/ f# P1 t; E
Rotation scores decreased gradually from 8186 ±2109 r/ min pre2transplantation to 4187 ±2106 r/ min 90 d post2
9 b7 {7 k3 g5 n8 stransplantation (statistic result showed P < 0105)
- ~5 G  e0 I9 o" l# m# w$ f3 Q; i) N7 P+ n2 ]( M
附件是pdf全文。感谢大家支持，回复本贴就可以下载了6 [* `0 O1 X8 A0 ?  [

* e8 K  u) P- Z- \' u( c2 Y

色色兔

先谢谢。再看

晓晓

谢！

laolifeidao999

非常感谢版主的分享，支持你

cz200203

5 p: A* J) V3 q; o) q- m
! [4 }! P, T0 U2 }
回复 1# cdk1
) S$ @  [$ j& l' ^7 r# Z6 A9 n+ k& L
( k) i( S: w9 v' J0 M
谢谢

sunshine226

Thanks

凤仪飘雪

零落幽谷

回复 1# cdk1
$ e2 s0 c3 L) j+ H/ q# [看看

tspaulzhao

Thanks.

weianh