外周血干细胞与组织工程骨移植修复犬牙周骨缺损区转化生长因子-β的表达

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赵淑贤,赵东方,王　敏,张建国,董　凯,张　兰
- |. H; g6 h; C& K; d[摘要] 　目的　探讨外周血干细胞(APBSC) 与组织工程骨(TEB) 复合移植修复牙周骨缺损中转化生长因子(TGF) -β的表达。
2 ?8 A' z9 T! ^5 G; p方法　犬的牙周骨缺损区移植APBSC - TEB 后,用免疫组织化学方法观察不同时期APBSC 分化、增殖成为成骨细胞过程中
" J# G4 }6 @0 w3 wTGF -β的表达,观察TGF -β诱导APBSC 分化、增殖成骨细胞的情况。结果　实验组在APBSC - TEB 移植2 周,即见骨缺损区
6 p+ T3 n+ b4 B" r+ V- v边缘TGF -β呈弱阳性表达,4～8 周骨缺损区中心区域可见大量的成骨细胞、成纤维细胞和胶原纤维呈现强阳性表达,12 周骨# u" L5 Z$ h! F& e
缺损区已完全修复。而对照组TEB 移植8～12 周,仅在骨缺损边缘区域TGF -β呈弱阳性反应。结论　APBSC 在骨缺损区分
" I  C+ {3 c, z1 v  |& J化、增殖为成骨细胞的过程中TGF -β是重要的调节因子,具有诱导成骨的作用。8 @# `1 t* Q0 ^9 v" k9 n# c# J. y# s
[关键词] 　外周血干细胞; 免疫组织化学; TGF -β表达; 犬3 K/ t7 p7 ~% t
[中图分类号] 　R781. 4 　　[文献标识码] 　A 　　[文章编号] 　100329872 (2007) 0520236203
4 _9 c3 ]5 c3 ?4 ^) u5 oExpression of TGF2β in region of dog experimental alveolar bone defect repaired by Transplantation APBSC2TEB4 {( L: l/ g8 N
ZHAO Shu2xian , ZHAO Dong2fang , WANG Min , ZHANG Jian2guo , DONG Kai , ZHANG Lan. ( Department of Oral Medicine , Stomatological
/ o% Q4 W+ F. [Hospital of Xi’an Jiaotong University , Xi’an 710004 , China)
2 H$ H3 e  Y- h" VAbstract : 　Objective 　To study the positive expression of TGF2βin the region of dog experimental alveolar bone defect repaired by Trans2
: H7 I- u  L2 S) Zplantation APBSC2TEB. Methods 　After the APBSC2TEB transplantation was introduced into the dog alveolar bone defect region ,the positive
/ M% _0 p9 C* gexpression of TGF2βof APBSC proliferation and differentiation into osteoblast was observed. Results 　After 2 weeks ,a weak2positive expression, z/ D6 K6 X0 M1 P+ ~4 [* p$ `
of TGF2B in the purlieus region of dog experimental alveolar bone defect was found. After 428 weeks ,the osteoblast ,collagenous fiber and fi2# r2 |& V% N# h
broblast showed strong2positive expression of TGF2βin the centre region of alveola bone defect in the experimental group. The alveolar bone de2
( [% R2 h8 b* ], |fect was repaired on the 12th week and only weak2positive expression of TGF2βin the purlieus region of alveolar bone defect was found in the8 `! J7 n8 r, K6 v, V
control group. Conclusion 　TGF2βis an important factor that promotes proliferation and differentiation of APBSC into osteoblast in the alveolar# x& O% D5 z$ t7 y1 Z% P
bone defect. TGF2βis effective on bone growth.) s/ Q7 N8 K0 i& `* I* Y! h
Key words : 　autologous periphera blood stem cell ; immunity tissue chemistry ; expression of TGF2β; dogs) N3 l3 \3 h: I* U
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绮梦

谢谢

luwei8157

该文献中，; Y- h; g$ x2 v! i
对外周血干细胞在移植区如何示踪？
' W0 T5 O0 b7 T; e1 f! S1 R$ T, b移植区TGF-b表达是机体对细胞的反应，还是细胞自身分化的表现？

巭秂

谢谢

dongxin

谢谢分享0 c  O: N1 n- Q; ~) ~( \! p