外周血干细胞与组织工程骨移植修复犬牙周骨缺损区转化生长因子-β的表达

cdk1

$ k2 y& C8 l, Y# U

, z, i8 S1 [* m* p7 {) P赵淑贤,赵东方,王　敏,张建国,董　凯,张　兰* L& {/ ^- q0 p7 r
[摘要] 　目的　探讨外周血干细胞(APBSC) 与组织工程骨(TEB) 复合移植修复牙周骨缺损中转化生长因子(TGF) -β的表达。
& {6 I9 c! O. y' X  K2 N4 K2 A方法　犬的牙周骨缺损区移植APBSC - TEB 后,用免疫组织化学方法观察不同时期APBSC 分化、增殖成为成骨细胞过程中4 u" S/ V& n( C# c* ^) I# A0 \
TGF -β的表达,观察TGF -β诱导APBSC 分化、增殖成骨细胞的情况。结果　实验组在APBSC - TEB 移植2 周,即见骨缺损区5 S* G0 i$ s2 P/ t
边缘TGF -β呈弱阳性表达,4～8 周骨缺损区中心区域可见大量的成骨细胞、成纤维细胞和胶原纤维呈现强阳性表达,12 周骨
, \% l- w8 n) \, l缺损区已完全修复。而对照组TEB 移植8～12 周,仅在骨缺损边缘区域TGF -β呈弱阳性反应。结论　APBSC 在骨缺损区分
7 c: v' i+ @8 P& u. j7 ]) @化、增殖为成骨细胞的过程中TGF -β是重要的调节因子,具有诱导成骨的作用。
2 O% w& D# T* J! c[关键词] 　外周血干细胞; 免疫组织化学; TGF -β表达; 犬
$ V+ F. N* l  T2 B( t4 a[中图分类号] 　R781. 4 　　[文献标识码] 　A 　　[文章编号] 　100329872 (2007) 0520236203
8 @3 L2 `$ |. v4 E7 @6 ^* [Expression of TGF2β in region of dog experimental alveolar bone defect repaired by Transplantation APBSC2TEB, N( o; J" z- L6 v- v
ZHAO Shu2xian , ZHAO Dong2fang , WANG Min , ZHANG Jian2guo , DONG Kai , ZHANG Lan. ( Department of Oral Medicine , Stomatological9 b4 R, R9 e: F
Hospital of Xi’an Jiaotong University , Xi’an 710004 , China)
' l# y; D7 n: e* z4 v5 h' rAbstract : 　Objective 　To study the positive expression of TGF2βin the region of dog experimental alveolar bone defect repaired by Trans2
% f! x, Z8 `& |' a* @* J9 ~plantation APBSC2TEB. Methods 　After the APBSC2TEB transplantation was introduced into the dog alveolar bone defect region ,the positive
6 S% _# h* ?' Oexpression of TGF2βof APBSC proliferation and differentiation into osteoblast was observed. Results 　After 2 weeks ,a weak2positive expression
* y' u) X& F2 R; Kof TGF2B in the purlieus region of dog experimental alveolar bone defect was found. After 428 weeks ,the osteoblast ,collagenous fiber and fi2
4 m* `1 O1 L9 Y* Jbroblast showed strong2positive expression of TGF2βin the centre region of alveola bone defect in the experimental group. The alveolar bone de2
2 g, T6 m% @8 X, q( H& p! u+ Qfect was repaired on the 12th week and only weak2positive expression of TGF2βin the purlieus region of alveolar bone defect was found in the
: W& K) a% q5 ?control group. Conclusion 　TGF2βis an important factor that promotes proliferation and differentiation of APBSC into osteoblast in the alveolar1 f5 g& }7 P- r* e" \5 _2 u: {
bone defect. TGF2βis effective on bone growth.% @9 C, a- v' k5 N& m* S
Key words : 　autologous periphera blood stem cell ; immunity tissue chemistry ; expression of TGF2β; dogs* l; Y1 C& ~4 S0 x- k+ _

# Z$ _. @5 \* t1 K$ Z5 I6 H5 |附件是pdf全文。感谢大家支持，回复本贴就可以下载了* C( [7 W6 a7 g, E/ G
" j& {0 x7 R- E

绮梦

谢谢

luwei8157

该文献中，
9 k  D) i: f$ a9 b对外周血干细胞在移植区如何示踪？
2 i& ?" q: H5 T移植区TGF-b表达是机体对细胞的反应，还是细胞自身分化的表现？

巭秂

谢谢

dongxin

谢谢分享" p5 @& [1 f, o. O6 I