诱导人脐血神经干细胞向神经细胞分化的研究

cdk1

& c0 _+ j  c: e2 S0 L: f
: e) \- w: [2 v3 q" H
季旭东1 , 高　超2 , 杨月景3 , 刘计荣4
) j3 D, R5 t3 W/ S4 {(11 郑州大学口腔医学院口腔内科　河南郑州　450052 ;21 郑州市儿童医院神经内科　河南郑州　450053 ;8 ]$ o5 X; q' ?5 P/ b2 a
31 郑州大学第二附属医院消化内科　河南郑州　450003 ;41 郑州大学基础医学院　河南郑州　450052)
! s. o1 I& D3 m" @6 o摘要: 目的: 体外诱导人脐血单个核细胞(mononuclear cells ,MNCs) 中的神经干细胞(neural stem cells ,NSCs) 向7 h4 H2 i$ |& y9 c( e* C- J2 @& U
神经细胞分化的研究。方法: 体外培养中用表皮生长因子(epithelium growth factor ,EGF) 和碱性成纤维细胞2 T3 e! @$ Y* p6 Y( E. F
生长因子(basic fibroblast growth factor ,bFGF) 诱导人脐血NSCs 向神经细胞的分化。结果: 在EGF 和bFGF 作
2 ^' |) K  d/ ]# Y用下,实验组Nestin 阳性细胞数量显著增多;在EGF 和bFGF 作用下,Nestin 的mRNA 表达PCR 条带变量两组& b* m$ L5 E# c3 o. \4 [
间有显著性差异。结论: 细胞因子EGF 和bFGF 在体外能促进脐血MNCs 中NSCs 的分化,联合应用较单独
7 ?$ O9 }/ J& Q- S; S' V$ L应用具有更强的促进脐血MNCs 向神经样细胞分化作用。
) Q: A9 h7 I6 e$ |关键词: 脐血; 神经干细胞; EGF; bFGF5 u8 y( |+ K$ W" k/ u% ^# H
中图分类号: R32912 　　文献标识码: A
; t& a& y! x1 t- q: Y) qInvestigation of differentiation derived from human umbilical  A% B1 |9 Q, X4 C
cord blood into neuron-like cells7 j' r5 ]; J" S" U' u6 R
J I Xu-dong1 , GAO Chao2 , YANG Yue-jing3 , LIU Ji- rong43 O8 c8 q4 V: i9 \+ `, f" l
( 1. Department of Oral Medicine , The College of Stomatology of Zhengzhou University , Zhengzhou 450052 , China ; 2. Department of Nerve
+ A) r1 H% \. f1 O3 EMedicine Chidren’s Hospital of Zhengzhou , Zhengzhou 450053 , China ; 3. Department of Digestion , The Secend Affiliated Hospital of* T" K9 b8 T) q$ |6 y
Zhengzhou University , Zhengzhou 450052 , China ; 4. Department of Pathophysiology , Basic Medical School of Zhengzhou University ,( [9 r8 F8 V! o0 ]6 {7 @" M" ]
Zhengzhou 450052 , China)
4 G+ n* f: s  _: ?  dAbstract : Objective : To investigate differentiation of nerve stem cells in mononuclear cells derived: N6 L" j% }( L5 J; j
from human umbilical cord blood into neuron-like cells in vitro. Methods : To induce the differentiation" S: X# ^$ Z1 |$ J' k) O
of nerve stem cells into neuron-like cells with epithelium growth factor ( EGF) and basic fibroblast( \: ~5 z2 g- \0 I2 U# `7 p
growth factor (bFGF) . Results : In the role of EGF and bFGF ,the nember of nestin’s positive cell is5 g* ?2 a# m( W1 e2 N
significant increasing in the experimental group. The belt of PCR which is expressed by nestin’s mRNA# g( }; W9 W- C6 D( N
is more brighter in experimental group ,there are significant difference in two groups. Conclusion : EGF
: C. H1 m, A8 i6 g9 fassociated with bFGF can improve the differentiation of nerve stem cells into neuron-like cells.7 j# m; u) G- w" O- l; A9 D
Keywords : mononuclear ; NSCs ; EGF ; bFGF. x# X- `& Q, C' B, s' U- L
* u8 r: K0 _, R* z6 h
附件是pdf全文。感谢大家支持，回复本贴就可以下载了
9 F) J7 d5 \4 S2 e! p3 G6 l' c0 R& w& C: q2 q4 B: J5 g$ Q0 B3 L