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- 积分
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- 威望
- 50
- 包包
- 191
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本书主要内容：/ ~* B3 ? T! \6 T9 ~7 Q7 h
1. Making Protein Immunoprecipitates
0 j+ ?' t, g3 ^7 u2. Signal Transduction Inhibitors in Cellular Function
* r' ]! Q6 r3 ^0 D) n( q3. Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets9 j' Y9 X5 {8 D& j
4. A High-Throughput Mammalian Cell-Based Transient Transfection Assay' X3 J0 P% J u' D- |* D( |
5. Determining Protein Half-Lives
7 | x9 P6 }+ L3 C* y. Z6. Assaying Protein Kinase Activity
3 J2 G# T6 f, O6 L6 }7. Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach! h2 @, N8 Q9 m& }& p
8. Studies of Calmodulin-Dependent Regulation
' v9 b1 P4 ^- |6 \, u) J9. Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
! |+ E0 q+ K- U1 h$ q4 ?3 l10. Characterization of Protein-DNA Assocation In Vivo by Chromatin Immunoprecipitation8 r7 c% ~- i4 `0 M. P% P. d* H9 H
11. Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
# }+ {3 K7 m& J8 ]+ y5 G( c* G4 M! u* h12. Nucleocytoplasmic Glycosylation, O-GlcNAc
! k) \9 P+ g. W E q5 e! x4 K13. Techniques in Protein Methylation
! U9 v* M/ r0 C+ i14. Assaying Lipid Phosphate Phosphatase Activities
2 Q+ J' v: ^$ e; G: }3 U4 U' i2 u5 ^' X15. Assaying Phosphoinositide Phosphatases% [3 p( P; _0 R: V! E5 X- h
16. Assaying Phospholipase A2 Activity
. ] g9 {! g8 R! ]5 O17. Measurement and Immunofluorescence of Cellular Phosphoinositides/ W# A& e: {% [3 E7 A
18. Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
( V! e* Q2 v9 T3 C) E2 W19. In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET% T% W" A3 D b
20. Revealing Protein Dynamics by Photobleaching Techniques: x7 _& C- J' P$ T! a, _% u
21. Assaying Cytochrome c Translocation During Apoptosis |
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总评分: 威望 + 6
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发表于 2010-7-14 12:12
