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本书主要内容:0 m5 G# W. B7 f
1. Making Protein Immunoprecipitates8 h+ y- [* ^0 ~1 m, ^6 C6 R
2. Signal Transduction Inhibitors in Cellular Function1 v: Q( i3 D" o$ L0 ?& }
3. Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets; X. H7 p( `$ I3 e
4. A High-Throughput Mammalian Cell-Based Transient Transfection Assay
) S# H. S3 T+ ~* B5. Determining Protein Half-Lives
2 A2 c$ _6 E; r; w3 {0 ^7 r8 f8 A6. Assaying Protein Kinase Activity
, g: D9 J3 y- b- d0 i* h7 v3 h7 D7. Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach
* l; {/ J6 i7 _: x8. Studies of Calmodulin-Dependent Regulation9 x7 J8 r0 t# g( t0 t
9. Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation. n1 Q* ?# M4 b6 Y6 _3 b% W
10. Characterization of Protein-DNA Assocation In Vivo by Chromatin Immunoprecipitation& b5 T* D, o! U- I/ Q
11. Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins+ O( z2 V7 i4 W N
12. Nucleocytoplasmic Glycosylation, O-GlcNAc8 a7 r7 l( C! X
13. Techniques in Protein Methylation3 C+ [9 d j0 O4 q) ]
14. Assaying Lipid Phosphate Phosphatase Activities
6 k7 Q: o, T7 d4 _' |* C15. Assaying Phosphoinositide Phosphatases
9 P8 o1 ?0 S% S4 W6 S1 X- b* z# s16. Assaying Phospholipase A2 Activity' {- h0 u+ G1 Q6 ~) T# M8 j' E7 R, ^; ?$ e
17. Measurement and Immunofluorescence of Cellular Phosphoinositides1 j$ I9 `+ F* q$ n% j% a
18. Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer) O3 B9 J3 J( u
19. In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
$ F, S W$ o2 N w) ~% A1 f/ c V20. Revealing Protein Dynamics by Photobleaching Techniques
: [4 r j/ j6 z+ d1 C% @21. Assaying Cytochrome c Translocation During Apoptosis |
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