请教精原干细胞相关问题

milklecherry

大家好，本人想请教：精原干细胞的研究前景如何？人精原干细胞的体外分离培养、纯化的难度是不是很大？存在哪些问题？目前有没有建立人的精原干细胞系？人精原干细胞诱导分化为精子样细胞难度大不大？本人刚涉及此领域，对此不大了解，敬请各位大侠指导！

yiwuya

研究前景如何？
) o& n* i& d: h& e8 \Male germline stem cells: from mice to men. Brinster RL. Science. 2007 Apr 20;316(5823):404-5. Review.0 i% n/ }' g1 u- a3 `1 S6 j
Germ cell research: a personal perspective. Yanagimachi R. Biol Reprod. 2009 Feb;80(2):204-18. Epub 2008 Dec 17. Review" }/ f3 A" z# A/ ?8 h

! l3 S: L4 K% \6 g9 E: x目前有没有建立人的精原干细胞系？
) f5 {7 M. |5 t, b6 q! I$ c, TPropagation of human spermatogonial stem cells in vitro. Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruji M, Hovingh S, de Reijke TM, de la Rosette JJ, van der Veen F, de Rooij DG, Repping S, van Pelt AM.JAMA. 2009 Nov 18;302(19):2127-34.5 r& z. r4 B, |8 K& E
with erratum in: JAMA. 2010 Feb 3;303(5):422.
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7 M$ r$ Z2 m4 l$ Q  k( R6 U人精原干细胞诱导分化为精子样细胞难度大不大？
7 ?6 @2 M! b, H+ I6 `5 Q: |4 oBelow is an unavailable cell line and unrepeatable work.
+ m, j  M1 p: q; \; x/ ]Generation and in vitro differentiation of a spermatogonial cell line.
3 }* ~% o, A# a. ]6 qFeng LX, Chen Y, Dettin L, Pera RA, Herr JC, Goldberg E, Dym M.Science. 2002 Jul 19;297(5580):392-5. Epub 2002 Jun 20.
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