请教精原干细胞相关问题

milklecherry

大家好，本人想请教：精原干细胞的研究前景如何？人精原干细胞的体外分离培养、纯化的难度是不是很大？存在哪些问题？目前有没有建立人的精原干细胞系？人精原干细胞诱导分化为精子样细胞难度大不大？本人刚涉及此领域，对此不大了解，敬请各位大侠指导！

yiwuya

研究前景如何？' K' g4 P9 G/ t7 S
Male germline stem cells: from mice to men. Brinster RL. Science. 2007 Apr 20;316(5823):404-5. Review.
; U1 K% j8 j0 W2 t4 MGerm cell research: a personal perspective. Yanagimachi R. Biol Reprod. 2009 Feb;80(2):204-18. Epub 2008 Dec 17. Review6 t' U) t: C8 v7 X! N

6 D9 U6 W2 E8 ]目前有没有建立人的精原干细胞系？
7 t: O9 _! m. ^- hPropagation of human spermatogonial stem cells in vitro. Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruji M, Hovingh S, de Reijke TM, de la Rosette JJ, van der Veen F, de Rooij DG, Repping S, van Pelt AM.JAMA. 2009 Nov 18;302(19):2127-34.
7 B7 \1 @* u/ Owith erratum in: JAMA. 2010 Feb 3;303(5):422. , A6 D. L! R# H

+ `1 L/ U$ q5 Q2 _& e人精原干细胞诱导分化为精子样细胞难度大不大？
$ k! Y" A' g& X1 n1 |Below is an unavailable cell line and unrepeatable work.
" o) r% m8 i3 o* bGeneration and in vitro differentiation of a spermatogonial cell line.
/ u! _. R. h* |Feng LX, Chen Y, Dettin L, Pera RA, Herr JC, Goldberg E, Dym M.Science. 2002 Jul 19;297(5580):392-5. Epub 2002 Jun 20.
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