请教精原干细胞相关问题

milklecherry

大家好，本人想请教：精原干细胞的研究前景如何？人精原干细胞的体外分离培养、纯化的难度是不是很大？存在哪些问题？目前有没有建立人的精原干细胞系？人精原干细胞诱导分化为精子样细胞难度大不大？本人刚涉及此领域，对此不大了解，敬请各位大侠指导！

yiwuya

研究前景如何？+ q8 X% n2 _- W' G0 ]
Male germline stem cells: from mice to men. Brinster RL. Science. 2007 Apr 20;316(5823):404-5. Review.* }( x& A7 g$ W. }) a
Germ cell research: a personal perspective. Yanagimachi R. Biol Reprod. 2009 Feb;80(2):204-18. Epub 2008 Dec 17. Review
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6 m- m) W1 M9 Y1 S8 \2 O目前有没有建立人的精原干细胞系？$ B. e8 r0 j: N% p8 o4 J
Propagation of human spermatogonial stem cells in vitro. Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruji M, Hovingh S, de Reijke TM, de la Rosette JJ, van der Veen F, de Rooij DG, Repping S, van Pelt AM.JAMA. 2009 Nov 18;302(19):2127-34.$ X! u: B8 Z: }0 W! p
with erratum in: JAMA. 2010 Feb 3;303(5):422. 6 d0 |  M' ]1 @4 r* s; D; q+ _
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人精原干细胞诱导分化为精子样细胞难度大不大？
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Generation and in vitro differentiation of a spermatogonial cell line.
, m$ i9 [8 j  f8 c) t+ [% tFeng LX, Chen Y, Dettin L, Pera RA, Herr JC, Goldberg E, Dym M.Science. 2002 Jul 19;297(5580):392-5. Epub 2002 Jun 20.
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Good luck