请教精原干细胞相关问题

milklecherry

大家好，本人想请教：精原干细胞的研究前景如何？人精原干细胞的体外分离培养、纯化的难度是不是很大？存在哪些问题？目前有没有建立人的精原干细胞系？人精原干细胞诱导分化为精子样细胞难度大不大？本人刚涉及此领域，对此不大了解，敬请各位大侠指导！

yiwuya

研究前景如何？
. v0 N6 N3 i& YMale germline stem cells: from mice to men. Brinster RL. Science. 2007 Apr 20;316(5823):404-5. Review.& i: P; _' j- h
Germ cell research: a personal perspective. Yanagimachi R. Biol Reprod. 2009 Feb;80(2):204-18. Epub 2008 Dec 17. Review) Z) E4 g, K/ J2 m: L
0 X( a: h, G* W5 C( @
目前有没有建立人的精原干细胞系？& z/ {( {2 s/ y7 Q6 X3 W, Y
Propagation of human spermatogonial stem cells in vitro. Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruji M, Hovingh S, de Reijke TM, de la Rosette JJ, van der Veen F, de Rooij DG, Repping S, van Pelt AM.JAMA. 2009 Nov 18;302(19):2127-34./ \' U. `0 Q5 n$ A
with erratum in: JAMA. 2010 Feb 3;303(5):422. # ]3 g2 {  s% `% x! T

8 y1 [7 x; t& X8 i( a. D7 K! I人精原干细胞诱导分化为精子样细胞难度大不大？
6 t% n' `1 V: x4 W% oBelow is an unavailable cell line and unrepeatable work.2 z$ J/ I  p$ V/ `! q
Generation and in vitro differentiation of a spermatogonial cell line.
4 z6 a8 w1 O; ~1 tFeng LX, Chen Y, Dettin L, Pera RA, Herr JC, Goldberg E, Dym M.Science. 2002 Jul 19;297(5580):392-5. Epub 2002 Jun 20.
4 I  g* L  I$ P* Q) v2 U1 }: ~9 ?( J" O
8 F8 i$ c- O- yGood luck