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本帖最后由 细胞海洋 于 2010-8-6 08:03 编辑 3 _1 w- L9 ~% L |' S- T
/ c- U K7 [. K2 _这是2010出版的 Methods in Molecular Biology 系列 vol.621# k" W4 y' t8 M' ?& c9 M
与本站朋友分享。
i% u/ s4 x& B5 t3 h（请勿外传，以免引起不必要的版权纠纷）
7 X- s$ e3 i( s' t6 x1 g8 j( ]2 ]& g3 ]
以下为该书前言：' q# L0 d% t+ v- _, J
Although embryonic stem cells currently enjoy the public limelight and show great promise
3 ]; n3 b$ x& W1 C& zfor cell based medical therapies, it is the adult stem cells which are responsible for the
/ I; |% @0 n$ Z y. \! C3 Q) Wbody’s natural ability to fight disease, heal and recover, or fail and succumb to various
& ?# s% w9 O5 H; }maladies. The study of mammalian adult stem cells has surged recently, most likely from a
2 d! w% g! L* R# m" S4 k9 V! i6 j! Hmaturation of stem cell studies in the classical developmental model organisms and in
x o7 U* O$ _- n1 b' x, G/ [! N4 Yhematopoeisis. All the tissues of the body examined so far are generated and regenerated" p f/ J0 F1 F9 z
from stem cells, it has been an important first step to adapt or devise new methods to
" \' p. |) R/ M% Eidentify and obtain these cells in quantity and purity for further study. Culture techniques! \: ~) s+ b. F+ E) `0 n
have been optimized for managing the growth and differentiation of stem cells in vitro; as9 D4 p& f% k, y( t6 s+ o
some stem cells are pluripotent, often the method is to guide the fate of such cells among
! p7 o& G8 }% w7 k/ d1 W: \the possible differentiation fates. Much of this work, and that in the classical model organisms,
- @* X' Z" q: L' x& ehas helped define the aspects of the stem cell environment or niche that are crucial: N6 T# \* d2 [: ^* t5 H q1 P- {6 i
for both growth and differentiation, and these studies have moved in vivo at increasingly
, Z, }9 M5 e9 W4 y6 c( y. O4 Ohigher resolution. Importantly, the in vivo niche is a current target for bioengineering the
7 [- l# H8 y; K* Tmatrix and signaling factors./ k& {0 t$ {7 f* U6 S
Herein, we present methods for studying six types of mammalian stem cells, mammary,1 ^* m; G+ }# ? j3 S7 _6 D
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches9 B6 p# f w: T# q2 ~* ^
described in these chapters will assist you in obtaining, characterizing, and studying these) P1 h; n+ C+ W: ^
cells or for adaptation to your favorite stem cells. One final note, should you find the+ A$ ~' f: [% J: A- c" Y$ t
information herein valuable and it helps you to make great discoveries in the understanding
' v2 U% ?( O, oof stem cell biology or application, by all means please let us, the authors, know! The
0 b e- N/ F; R7 x0 Q% ebest way is of course to publish your findings, but falling short of that, please feel free to2 Y4 t& y9 Z6 @7 L0 e" F6 p
ask questions or clarifications. Especially for this reason, our contact information is provided, O# r3 y( m8 d& h2 I, J* H2 m/ N% r
at the end of this book." q, Q0 T/ ~8 T" L% o# m; {
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发表于 2010-8-5 17:09
