PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

jimmy88cn

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这是2010出版的 Methods in Molecular Biology 系列 vol.621
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（请勿外传，以免引起不必要的版权纠纷）
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# \' h" }' e: Y' i; X$ a- m以下为该书前言：
' X, j' _! O" T7 R/ n3 MAlthough embryonic stem cells currently enjoy the public limelight and show great promise
4 C! K/ F# m' tfor cell based medical therapies, it is the adult stem cells which are responsible for the
( {+ n% i7 t2 e; W0 z- Z* F; obody’s natural ability to fight disease, heal and recover, or fail and succumb to various
/ Q# Q: x$ G& @$ P0 M6 Hmaladies. The study of mammalian adult stem cells has surged recently, most likely from a( ~# p# U" p# F' e" G6 F/ e
maturation of stem cell studies in the classical developmental model organisms and in
4 k5 E# q' @8 O) A& [7 k: shematopoeisis. All the tissues of the body examined so far are generated and regenerated& o% r6 P/ {- }( F8 n; ~% s6 G4 ?/ [. z
from stem cells, it has been an important first step to adapt or devise new methods to
4 L. f, E& x2 g" T! }0 Aidentify and obtain these cells in quantity and purity for further study. Culture techniques2 k- C+ f% q) O1 Z
have been optimized for managing the growth and differentiation of stem cells in vitro; as4 d5 e/ o; H/ _4 h6 Y' c
some stem cells are pluripotent, often the method is to guide the fate of such cells among* A7 _' {7 u% Z1 n- a: q
the possible differentiation fates. Much of this work, and that in the classical model organisms,  {& [) k8 @' {2 F$ _: }0 ^
has helped define the aspects of the stem cell environment or niche that are crucial
: u) Z7 L) t) K0 V& a' Rfor both growth and differentiation, and these studies have moved in vivo at increasingly
- i" b5 O. S+ [( W$ uhigher resolution. Importantly, the in vivo niche is a current target for bioengineering the
9 f9 O* A$ T& w$ rmatrix and signaling factors.
4 z$ d* m" d6 [) t/ ^' PHerein, we present methods for studying six types of mammalian stem cells, mammary,5 x8 j5 ^; v9 ]8 G
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches  E# K. @$ J  N( T+ P# ]
described in these chapters will assist you in obtaining, characterizing, and studying these
- R6 V: |0 c0 A. P# @cells or for adaptation to your favorite stem cells. One final note, should you find the" ~, {$ Q0 P% R, g9 b, t+ F. \
information herein valuable and it helps you to make great discoveries in the understanding
5 |+ |" ~. k; Sof stem cell biology or application, by all means please let us, the authors, know! The* c! {$ h% H: k
best way is of course to publish your findings, but falling short of that, please feel free to: b3 j6 Z$ u7 r( a# _
ask questions or clarifications. Especially for this reason, our contact information is provided  v1 W& b6 i# M8 I% ~& h  ]
at the end of this book.6 `8 ^$ S+ v1 l, j
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