PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

jimmy88cn

. T( h$ }# V& z* t: T; c* Y( f9 z. _, x$ V
这是2010出版的 Methods in Molecular Biology 系列 vol.621
. F* ]. L' n) b  k6 n# v与本站朋友分享。, D( x2 Y5 X8 |
（请勿外传，以免引起不必要的版权纠纷）
  G3 H6 N4 P8 z5 n6 B6 i1 z6 u
$ r4 {( a. B$ K( f  H0 A以下为该书前言：
: r5 \" v9 Q% C! U5 q8 @Although embryonic stem cells currently enjoy the public limelight and show great promise7 O2 b( }1 a' k6 ?( A
for cell based medical therapies, it is the adult stem cells which are responsible for the& }0 |; J- s- F, K2 p4 d7 x
body’s natural ability to fight disease, heal and recover, or fail and succumb to various* C; m* N  ~0 |5 `2 O6 Z8 ^- x9 V
maladies. The study of mammalian adult stem cells has surged recently, most likely from a6 m* O+ b# L% N7 _1 s! w
maturation of stem cell studies in the classical developmental model organisms and in4 {; o8 T4 {7 e! z2 @) S* P
hematopoeisis. All the tissues of the body examined so far are generated and regenerated
* ^$ c5 c  ^1 ^# h$ H: ^from stem cells, it has been an important first step to adapt or devise new methods to9 E& Y$ g! `: S0 Z
identify and obtain these cells in quantity and purity for further study. Culture techniques
& \! X+ E! T, h% \6 ?) mhave been optimized for managing the growth and differentiation of stem cells in vitro; as" B6 E) b1 F6 o# ?& l0 a2 G
some stem cells are pluripotent, often the method is to guide the fate of such cells among3 z( v" o# k2 [% K, g9 @, ~% }
the possible differentiation fates. Much of this work, and that in the classical model organisms,  f- m  v; I( ~  G* K$ p
has helped define the aspects of the stem cell environment or niche that are crucial7 q" l! x& v, X" G' f( |, R) s; z
for both growth and differentiation, and these studies have moved in vivo at increasingly! q! q/ |: g, G- F" M& E3 O
higher resolution. Importantly, the in vivo niche is a current target for bioengineering the3 i2 P' Y' I  W; |" k( |
matrix and signaling factors.* K: X5 V! K7 F
Herein, we present methods for studying six types of mammalian stem cells, mammary,
( q5 c& o% w* e4 c$ H$ v8 tneural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches' j" p; S0 j8 Q# {7 K
described in these chapters will assist you in obtaining, characterizing, and studying these% R9 O3 U5 x( L+ V- K, H
cells or for adaptation to your favorite stem cells. One final note, should you find the
7 c- m( }; ?5 W& Ginformation herein valuable and it helps you to make great discoveries in the understanding7 K1 W) C7 G2 T
of stem cell biology or application, by all means please let us, the authors, know! The$ |, @6 V/ N9 m* B
best way is of course to publish your findings, but falling short of that, please feel free to
6 _$ m. P, j2 y. v1 i3 Kask questions or clarifications. Especially for this reason, our contact information is provided  s$ i2 R9 w, T" I7 ?! F
at the end of this book.) `5 `0 d& ^, K% M
6 a! f2 i0 X1 j5 [" |1 s/ w7 _
附件为ｐｄｆ全文　，回复本贴就可以下载了" G1 o9 ?( u5 Y8 h8 c- }
* Z+ |1 S3 Z- _# G5 T# d/ I' ^
[hide][/hide]

tspaulzhao

Thanks.

zoe

多谢分享

冠忠

回复 1# jimmy88cn
6 {1 N& M. B. f, J  p1 p; h
$ v/ }, [& y0 q. O6 R, L5 ?$ D, P, E8 _2 e  r' x& C2 Y/ P% F
很好呀

woosheng

谢谢

wgydys

回复 1# jimmy88cn
/ N: B$ E' [, o! \& r' }! O! T
5 D' ], U5 O- E9 V' F4 o0 m/ e5 [8 J  G5 X
    thanks

botizhou

Thanks.

yibo10679

谢谢

yibo10679

谢谢

恒宇

thanks