PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

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# y9 u, v' D: J; k这是2010出版的 Methods in Molecular Biology 系列 vol.621" t+ S: z+ D6 b* A+ v+ Q6 l: Y
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5 I5 R. @9 N) g, w5 L7 }8 r以下为该书前言：8 ~4 m1 I; U$ q. T  b6 n
Although embryonic stem cells currently enjoy the public limelight and show great promise) N1 n, W) V1 j' c0 C/ _) H  `
for cell based medical therapies, it is the adult stem cells which are responsible for the
1 A/ V1 `& P7 r7 Y( B+ O) g9 D  vbody’s natural ability to fight disease, heal and recover, or fail and succumb to various8 e3 B% b8 h8 w. o. D3 y& o4 `; B8 y
maladies. The study of mammalian adult stem cells has surged recently, most likely from a
6 v4 u2 O) {" b6 u0 X  lmaturation of stem cell studies in the classical developmental model organisms and in9 q) D: z8 z) u) q
hematopoeisis. All the tissues of the body examined so far are generated and regenerated: u% N' T6 L6 Y7 V6 @- D
from stem cells, it has been an important first step to adapt or devise new methods to
1 s, Y2 f& e4 Y1 J& O: Bidentify and obtain these cells in quantity and purity for further study. Culture techniques
5 ~& V8 e' q! V0 Ghave been optimized for managing the growth and differentiation of stem cells in vitro; as
1 M$ e: a' Z8 z" X# }some stem cells are pluripotent, often the method is to guide the fate of such cells among
" R6 H, m6 W) H0 ^the possible differentiation fates. Much of this work, and that in the classical model organisms,
& d* V2 a' W2 L3 whas helped define the aspects of the stem cell environment or niche that are crucial0 T& |/ ^  s7 m: E* O
for both growth and differentiation, and these studies have moved in vivo at increasingly) M3 q; H1 y: y' H9 D5 a/ z
higher resolution. Importantly, the in vivo niche is a current target for bioengineering the- \8 u0 Q( X  v3 S& T
matrix and signaling factors.
# S  ^+ E& c4 L$ z" e# F4 oHerein, we present methods for studying six types of mammalian stem cells, mammary,; y' Q: @: j- \0 L) d0 B8 R, g+ a
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches9 D$ ], s' D; X8 k8 d8 k, u6 [4 J
described in these chapters will assist you in obtaining, characterizing, and studying these5 W* c! d$ C- u0 t: v! [# g& p/ g, b
cells or for adaptation to your favorite stem cells. One final note, should you find the
% T: b  ], j* ^9 q2 ?9 k$ winformation herein valuable and it helps you to make great discoveries in the understanding
; L$ J3 z: _% m$ n% zof stem cell biology or application, by all means please let us, the authors, know! The- L* I/ `# }' [: N
best way is of course to publish your findings, but falling short of that, please feel free to  W/ }! H" b2 I. h) J2 l
ask questions or clarifications. Especially for this reason, our contact information is provided
6 X% G( Y% U9 X0 Uat the end of this book.
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