PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

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8 }8 E3 X, g3 c4 C8 |. P* z这是2010出版的 Methods in Molecular Biology 系列 vol.621
, E& S/ F5 D5 F& \3 R4 O6 U- h与本站朋友分享。
0 |3 Q6 V* w" |5 [- Z( W% l（请勿外传，以免引起不必要的版权纠纷）
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2 ]" ]  K/ R6 [6 e以下为该书前言：
7 R/ {& l& Y  N. \( T5 wAlthough embryonic stem cells currently enjoy the public limelight and show great promise& q/ _( r# [/ Y
for cell based medical therapies, it is the adult stem cells which are responsible for the3 y1 ~" x4 t- @! c. \0 q
body’s natural ability to fight disease, heal and recover, or fail and succumb to various
) G! G1 l7 _5 S# z* mmaladies. The study of mammalian adult stem cells has surged recently, most likely from a/ k7 k8 m2 A2 b/ _7 l1 m7 B6 U& n
maturation of stem cell studies in the classical developmental model organisms and in
1 c4 C+ v4 |' M; D6 G. |* }hematopoeisis. All the tissues of the body examined so far are generated and regenerated- v6 z) O' E) n: E- _. v
from stem cells, it has been an important first step to adapt or devise new methods to
  j' Z) s- _) x" l7 z8 V/ ]) ^  Yidentify and obtain these cells in quantity and purity for further study. Culture techniques8 j/ \$ V; ?+ ~
have been optimized for managing the growth and differentiation of stem cells in vitro; as
5 E' @8 _& q$ y6 w) x4 psome stem cells are pluripotent, often the method is to guide the fate of such cells among
2 @/ n! e9 A+ |! S, d; ethe possible differentiation fates. Much of this work, and that in the classical model organisms,* u6 p0 C% p, w
has helped define the aspects of the stem cell environment or niche that are crucial
$ h4 H0 R9 n7 q" {$ lfor both growth and differentiation, and these studies have moved in vivo at increasingly9 y! {7 Y" L6 k+ ?% f. J
higher resolution. Importantly, the in vivo niche is a current target for bioengineering the
& s1 ?7 u5 S# r  c: r! Ematrix and signaling factors.+ E; |4 V" M, B/ e
Herein, we present methods for studying six types of mammalian stem cells, mammary,
4 n! ]& h7 ~1 d  |5 y4 y" yneural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches
' @2 M! A% d5 o3 h7 V& k8 ]described in these chapters will assist you in obtaining, characterizing, and studying these
3 \8 j- G! F) \$ zcells or for adaptation to your favorite stem cells. One final note, should you find the2 ]3 `& f4 J2 B' \' I
information herein valuable and it helps you to make great discoveries in the understanding
/ \6 ]! D- K7 d0 Z+ {of stem cell biology or application, by all means please let us, the authors, know! The1 r7 `5 k; X2 |( ]; _" S! M: P0 f# ]6 s
best way is of course to publish your findings, but falling short of that, please feel free to
6 E5 B( @$ e( T7 m4 j. Aask questions or clarifications. Especially for this reason, our contact information is provided
8 h1 h6 q* Q# X% u% V3 \at the end of this book.
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