PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

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这是2010出版的 Methods in Molecular Biology 系列 vol.621
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以下为该书前言：4 m' U& k+ _, q4 r5 F. Z6 ~
Although embryonic stem cells currently enjoy the public limelight and show great promise/ s3 g' _  N! x8 H
for cell based medical therapies, it is the adult stem cells which are responsible for the
. A' ]5 W, _! j" `body’s natural ability to fight disease, heal and recover, or fail and succumb to various6 k& a+ \6 ?! A$ j. g- W/ N+ f
maladies. The study of mammalian adult stem cells has surged recently, most likely from a, v; v) m$ u/ P2 h" h' V
maturation of stem cell studies in the classical developmental model organisms and in
. u9 @2 o# t/ E& shematopoeisis. All the tissues of the body examined so far are generated and regenerated2 K" V6 N0 \- v# K' Q; @' Y
from stem cells, it has been an important first step to adapt or devise new methods to* C3 y, ?* Y. [+ c
identify and obtain these cells in quantity and purity for further study. Culture techniques  c/ t: |5 X- |' t, C
have been optimized for managing the growth and differentiation of stem cells in vitro; as
3 H  q- A& W2 G/ d+ [* F) lsome stem cells are pluripotent, often the method is to guide the fate of such cells among/ V5 }. f+ S- Q' h! j
the possible differentiation fates. Much of this work, and that in the classical model organisms,: a( X* f: `! ?$ [
has helped define the aspects of the stem cell environment or niche that are crucial* H2 w& U, m7 j2 H, n
for both growth and differentiation, and these studies have moved in vivo at increasingly3 \/ Z- v/ `( c* K! [2 t  z
higher resolution. Importantly, the in vivo niche is a current target for bioengineering the8 V1 E# ?# _6 c9 F+ T
matrix and signaling factors." Z& b# P5 ]4 _. `  f7 f
Herein, we present methods for studying six types of mammalian stem cells, mammary,
/ O- X- s* L9 U! D3 V; C/ p7 cneural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches
! D( U1 W+ h& P$ B2 I) w6 Xdescribed in these chapters will assist you in obtaining, characterizing, and studying these
2 Z$ G" j3 ?: \& Vcells or for adaptation to your favorite stem cells. One final note, should you find the
' W3 a; R& z7 x, O+ sinformation herein valuable and it helps you to make great discoveries in the understanding/ l+ f2 ^1 F4 v" z* u- O$ U! @
of stem cell biology or application, by all means please let us, the authors, know! The
  a9 i  [: j5 f* _6 v+ fbest way is of course to publish your findings, but falling short of that, please feel free to
$ _- R7 i: T1 A7 a* ~2 \- f- Eask questions or clarifications. Especially for this reason, our contact information is provided
# j2 _5 Y7 R  {" o% Q3 z# Iat the end of this book.
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